Background: White birch and hazel allergens, namely Bet v1 Cor a1 are known but their allergen specificity is not yet characterized.
 Objective: To map the antigenic determinants responsible for IgE binding utilizing in silico modelling docking of peptides against antibody.
 Methods: The antigen sequences were cut into docked antibody those with highest scores further studied bond int...

Prolonged flurazepam exposure regulates the expression of selected (a1, b2, b3) GABAA receptor subunit messenger RNAs in specific regions of the hippocampus and cortex with a time-course consistent with benzodiazepine tolerance both in vivo and in vitro. In this report, the immunostaining density of six specific GABAA receptor subunit (a1, a2, b1–3 and g2) antibodies was measured in the hippoca...

BACKGROUND Botulism is a naturally occurring disease, mainly caused by the ingestion of food contaminated by the botulinum neurotoxins (BoNTs). Botulinum neurotoxins are the most lethal. They are classified among the six major biological warfare agents by the Centers for Disease Control. BoNTs act on the cholinergic motoneurons, where they cleave proteins implicated in acetylcholine vesicle exo...

■ ■ Excellent linearity, accuracy, and precision INTRODUCTION Apolipoprotein A1 (Apo A1) protein is a major component of High Density Lipoprotein (HDL) in plasma. Along with other lipoproteins, Apo A1 plays a key role in lipid metabolism.1 While typical levels of Apo A1 are >1.2 mg/mL for males and >1.4 mg/mL for females,2 reduced plasma concentrations of Apo A1 have been reported in patients a...

We determined the crystal structures of the T cell receptor (TCR)-like antibody 25-D1.16 Fab fragment bound to a complex of SIINFEKL peptide from ovalbumin and the H-2K(b) molecule. Remarkably, this antibody directly "reads" the structure of the major histocompatibility complex (MHC)-bound peptide, employing the canonical diagonal binding mode utilized by most TCRs. This is in marked contrast w...

BACKGROUND AND PURPOSE Although the 'cromones' (di-sodium cromoglycate and sodium nedocromil) are used to treat allergy and asthma, their 'mast cell stabilising' mechanism of pharmacological action has never been convincingly explained. Here, we investigate the hypothesis that these drugs act by stimulating the release of the anti-inflammatory protein Annexin-A1 (Anx-A1) from mast cells. EXPE...

A total of 76 blood samples from patients without malignant disease and 107 blood samples from patients with malignant disease were investigated for the presence of circulating tumor cells (CTCs). To detect CTCs, hematopoietic cells were removed from the blood samples and different RNA extraction methods were used to amplify the melanoma antigen-encoding gene family member A1-family member A6 (...

The new piericidin group antibiotics, glucopiericidins A and B were isolated from the culture broth of Streptomyces pactum S48727 (FERM P-8117) as co-metabolite of piericidin A1. The structures of glucopiericidins A and B were determined as piericidin A1, 10-O-beta-D-glucoside and piericidin A1, 3'-O-D-glucoside on the basis of their spectral and chemical properties, respectively. Glucopiericid...

This study was conducted to investigate the effects of protexin probiotic and aquablend avian antibody on the performance and immune system of broilers. In this experiment, 320 1-day-old male broiler chicks of the Ross 308, with 5 treatments and 4 replications in a completely randomized design were distributed in experimental units (cages). The treatments include: control (C), protexin (P1), pr...