background: tuberculosis (tb) remains as a major cause of death around the world. construction of a new vaccine against tuberculosis is an effective way to control it. several vaccines against this disease have been developed. the aim of the present study was to cloning of tb10.4 gene in pcdna3.1+ plasmid and evaluation of its expression in eukaryotic cells. methods: firstly, tb10.4 fragment wa...

The families of TGF-β proteins are the most important growth factors in the ovary for growth and differentiation of early ovarian follicles. Three related oocyte-derived members of the transforming growth factor-β superfamily namely growth differentiation factor 9 (GDF9), BMP15 and BMPR-IB have been shown to be essential for follicular growth and ovulation. Different mutations in the GDF9 gene ...

mycosphaerella graminicola, the causal agent of septoria tritici blotch (stb), is a widespread and significant pathogen of wheat. to determine mating types, 89 isolates were collected randomly from wheat fields of khuzestan, east azerbaijan, ardebil, kermanshah and golestan provinces of iran, during 2006-7. dna was extracted based on standard protocols. multiplex pcr was conducted using two pai...

a rapid and sensitive reverse transcription polymerase chain reaction (rt-pcr) and nested-pcr were used to detect bovine viral diarrhea virus 1 (bvdv-1) in bull semen. selected primers could amplify a part of the 5´utr of the bvdv genome. a 294 bp dna fragment was amplified and specificity of the results was confirmed by direct sequencing of the pcr product. prior to rna extraction, the seminal...

expressions of recombinant proteins for different applications are important objectives in molecular biotechnology; however, expression of some recombinant proteins is difficult. several methods have been designed for expression of these proteins. the aim of this study was to construct a vector containing mtb32c fragment of mycobacterium tuberculosis (m.tuberculosis) as a fusion partner in orde...

the families of tgf-β proteins are the most important growth factors in the ovary for growth and differentiation of early ovarian follicles. three related oocyte-derived members of the transforming growth factor-β superfamily namely growth differentiation factor 9 (gdf9), bmp15 and bmpr-ib have been shown to be essential for follicular growth and ovulation. different mutations in the gdf9 gene ...

Panulirus argus Virus 1 (PaV1) is a pathogenic virus that infects Caribbean spiny lobsters P. argus in the Florida Keys. We have developed a PCR detection assay for PaV1 for the purpose of studying the natural history of the virus and for monitoring the prevalence of infection. The detection of the virus in hemolymph and other tissues is based on the PCR amplification of a 499 bp product using ...

we describe a rapid, simple, and reliable procedure for routine isolation of mycobacterium leprae dna from slit-skin swab specimens. this one-step dna extraction method is based on the utilization of chelexr 100, a chelating ion exchange resin. slit-skin swab specimens from seven untreated leprosy patients at baba baghi leprosy hospital in iran were processed by this procedure. the polymerase c...