rospectively. However, for years, retrospective reports of pregnancy wantedness have been recognized to be subject to recall error or rationalization. As long as three decades ago, it was noted that respondents might not admit to what could be perceived as an inadequacy, or that they might change their attitude based on positive experiences with a child resulting from a pregnancy that, at conce...

NATURE | VOL 410 | 8 MARCH 2001 | www.nature.com 227 12. DePace, A. H., Santoso, A., Hillner, P. & Weissman, J. S. A critical role for amino-terminal glutamine/ asparagine repeats in the formation and propagation of a yeast prion. Cell 93, 1241–1252 (1998). 13. Patino, M. M., Liu, J. J., Glover, J. R. & Lindquist, S. Support for the prion hypothesis for inheritance of a phenotypic trait in yeas...

Advanced single-cell analysis technologies (e.g., mass cytometry) that help in multiplexing cellular measurements in limited-volume primary samples are critical in bridging discovery efforts to successful drug approval. Mass cytometry is the state-of-the-art technology in multiparametric single-cell analysis. Mass cytometers (also known as cytometry by time-of-flight or CyTOF) combine the cellu...

Methanosarcina barkeri 227 possesses two clusters of genes potentially encoding nitrogenases. We have previously demonstrated that one cluster, called nif2, is expressed under molybdenum (Mo)-sufficient conditions, and the deduced amino acid sequences for nitrogenase structural genes in that cluster most closely resemble those for the Mo nitrogenase of the gram-positive eubacterium Clostridium ...

A simple and sensitive ultraviolet spectrophotometric method for quantitative estimation of glibenclamide in presence of lipid turbidity has been developed, to avoid false estimation due to diffraction by turbidity. UV detection has been performed at 232 nm, 227 nm and 237 nm and the calibration curve is plotted between resultant of absorbance of [232 nm – (227 nm + 237 nm)/2] and concentration...

Designing potent silencing triggers is key to the successful application of RNA interference (RNAi) in mammals. Recent studies suggest that the assembly of RNAi effector complexes is coupled to Dicer cleavage. Here we examine whether transfection of optimized Dicer substrates results in an improved RNAi response. Dicer cleavage of chemically synthesized short hairpin RNAs (shRNAs) with 29-base-...

What’s the best thing about HHMI funding? There's lots of it. The annual HHMI budget was $330 million in 1994, of which about 80 % was spent on the 227 HHMI investigators. For those who need lots of money for mice or equipment, HHMI funding can be the answer to a prayer. And HHMI, unlike most other funding bodies, does not discourage long-term, risky research or projects with little obvious rel...