Acid phosphatase from yeast Saccharomyces cerevisiae was purified, and its physicochemical and kinetic properties were investigated. The sedimentation coefficient has been determined to be s0(20,w) = 13.6 S. The diffusion constant has been found to be 3.9 X 10(-7) cm2s-1, and the calculated partial specific volume was v = 0.663 cm3/g. From these data, a molecular weight of 252,000 was calculate...

Ca efflux in dialyzed squid axons was measured with 45Ca as a function of internal ionized Ca in the range 0.005-10 muM. Internal Ca stores were depleted by treatment with CN and dialysis with media free of high energy compounds. The [Ca]iota was stabilized with millimolar concentrations of EDTA, EGTA, or DTPA. Nonspecific leak of chelated Ca was measured with [14C]-EDTA and found to be 0.02 pm...

Abstract We present a distant T-type brown dwarf candidate at ≈2.55 kpc discovered in the Cosmic Evolution Early Release Science (CEERS) fields by James Webb Space Telescope (JWST) NIRCam. In addition to superb sensitivity, we utilised 7 filters from JWST near-IR and thus is advantageous finding faint, previously unseen dwarfs. From model spectra new JWST/NIRCam filter wavelengths, selection cr...

The objective of this study was to improve the overall performance a glassy carbon electrode (GCE) for detection 2,6-diaminotoluene (TDA), possibly carcinogenic primary aromatic amines (PAAs) that poses serious risk consumer’ health because they can transfer from multilayer food packages including adhesives based on polyurethane (PU) systems, food. modification surface made by means multi-walle...