A radioimmunoassay for bleomycin has been produced using 125l-labeled bleomycin and antisera raised in rabbits against a carbodiimide-catalyzed bleomycin-bovine serum albumin conjugate. 125l-Labeled bleomycin was synthesized by direct iodination of the drug using the chloramine-T technique. The standard curve of the assay was linear on a logit-log plot and the lower limit of sensitivity was 250...

The effect of sequence and timing of hyperthermia (43 degrees) and bleomycin on Chinese hamster cells (V79) has been investigated. Hyperthermia preceding bleomycin treatment produced a greater cytotoxic effect than bleomycin treatment preceding hyperthermia. Furthermore, it appears that the combination of hyperthermia and bleomycin becomes less effective if the application of bleomycin is delay...

Combined therapy with clarithromycin (CLTR) and N–acetyl cysteine (NAC) may be useful in diseases with impaired oxidant-antioxidant balance, fibroblast proliferation, and collagen deposition such as pulmonary fibrosis. Activated inflammatory cells which accumulate in the lower airways may release increased amounts of reactive oxygen species (ROS) when accompanied with a deficiency in glut...

    Many studies have been performed for treatment or prevention of pulmonary fibrosis. However, no effective treatment has been found yet. The aim of this study was to investigate the effect of grape seed extract on bleomycin-induced lung fibrosis in rat. Hydroalcoholic extract of grape seed (Vitis vinifera) was prepared using maceration method. NMRI rats weighing 250-300 g were given...

Background and aim: Warts are the most common clinical presentation of human papilloma virus infection of human skin and mucosa. They are more common on hand, foot, face and genital area. They are benign skin colour lesions with different clinical forms. There is no specific treatment for them. In this trial the therapeutic effect of intralesional injection of bleomycin and cryotherapy on...

Bleomycin was administered to six patients with advanced cancer. Multiple parameters of both antibody- and cell-mediated immunity were followed serially to characterize the effects of bleomycin on immunocompetence in humans. Antibody-mediated immunity, including primary vaccination with keyhole limpet hemocyanin, was not depressed. While there was no significant suppression of cell-mediated imm...

Tallysomycin is an antitumor antibiotic that is structurally related to bleomycin. The mechanism of action of tallysomycin appears to be similar to that of bleomycin. Bleomycin induces strand scission of purified isolated DNA [ l--5]. Bleomycin has also been shown to produce breakage of intracellular DNA [5,6]. Iron(H) appears to be associated with the mechanism of action of bleomycin. The foll...

Bleomycin was administered to six patients with advanced cancer. Multiple parameters of both antibodyand cell-mediated immunity were followed serially to characterize the effects of bleomycin on immunocompetence in humans. Antibody-mediated immunity, including primary vaccination with keyhole limpet hemocyanin, was not depressed. While there was no significant suppression of cell-mediated immun...

Lung fibrosis is a common side effect of the chemotherapeutic agent, bleomycin. Current evidence suggests that reactive oxygen species may play a key role in the development of lung fibrosis. The present study examined the effect of mesna on bleomycin-induced lung fibrosis in rats. Animals were divided into three groups: (1) saline control group; (2) Bleomycin group in which rats were injected ...