Treatment of calf thymus DNA with sodium bisulfite gave no deamination of cytosine to uracil, under conditions where 68 % of the cytosines of heat-denatured DNA were converted to uracil. This implies that the mutagenic properties of bisulfite are due to reaction with single-stranded DNA. Similarly, poly(1) . poly(C) was unreactive to bisulfite under conditions where poly(C) reacted readily. It ...

the interaction of p-sulphonatocalix [6] arene towards l-arginine and l-lysine has been studied inacidic aqueous solution (p1-1 2) using uv-vis spectrophotometric titration technique. the psulphonatocalix[6] arene was found to be able to form 1:1 complexe with amino acids in water.the values of the association constant were determined at different temperatures to evaluate thethermodynamic funct...

The title compound, C(7)H(13)NO(3), adopts an approximately planar conformation. The torsion angles in the aliphatic chain between the carbonyl group C atoms range from 172.97 (14) to 179.38 (14)° and the r.m.s. deviation of all non-H atoms is 0.059 Å. The crystal packing is dominated by two strong N-H⋯O hydrogen bonds involving the amide groups and forming R(2) (2)(8) rings and C(4) chains. Ov...

The peculiar versatility of remotely enolizable 6-methyluracil-5-carbaldehydes as useful vinylogous pronucleophiles in direct, asymmetric [4+2] cyclizations with suitable nitroolefins has been demonstrated. Under the strategic exploitation noncovalent bifunctional organocatalysis, a dearomative remote enolization strategy was implemented, to generate oQDM-type dienolate intermediates that were ...

Multiply damaged sites (MDS) are defined as greater than/equal to two lesions within 10-15 bp and are generated in DNA by ionizing radiation. In vitro repair of closely opposed base damages > or =2 bp apart results in a double strand break (DSB). This work extends the in vitro studies by utilizing clusters of uracil DNA damage as model lesions to determine whether MDS are converted to DSBs in b...

UV irradiation of 1,3-dimethyluracil in ice-matrix yields four dimeric dimethyluracils (2 c) — (5 c) , which could be isolated by preparative thin-layer chromatography. Attemps for structure elucidation of these four dimers by UV, IR, NMR and mass-spectrometric methodes pointed out, that only NMR-spectra give structural evidences; by IR — not by mass-spectra — certain identification of dimers i...

1-alkoxymethyl-5-alkyl-6-naphthylmethyl uracils, which are novel 1-[(2-hydroxyethoxy)methyl]-6-(phenylthio)thymine (HEPT) analogues, were synthesized for evaluation as selective and potent nonnucleoside human immunodeficiency virus (HIV)-1 reverse transcriptase inhibitors. The anti-HIV-1 activity of these compounds was assayed in vitro using HIV-1 infected MT-4 and CEM bioassays. The EC50, CC50...