Electron paramagnetic resonance (EPR) studies of succinate:ubiquinone oxidoreductase (SQR) from Paracoccus denitrificans have been undertaken in the purified and membrane-bound states. Spectroscopic "signatures" accounting for the three iron-sulfur clusters (2Fe-2S, 3Fe-4S, and 4Fe-4S), cytochrome b, flavin, and protein-bound ubisemiquinone radicals have been obtained in air-oxidized, succinate...

Investigations were performed with regard to the function of the iron-sulfur cluster of L-serine dehydratase from Peptostreptococcus asaccharolyticus, an enzyme which is novel in the class of deaminating hydro-lyases in that it lacks pyridoxal-5'-phosphate. Anaerobically purified L-serine dehydratase from P. asaccharolyticus revealed EPR spectra characteristic of a [3Fe-4S]+ cluster constitutin...

The NADH-quinone oxidoreductase from Paracoccus denitrificans consists of 14 subunits (Nqo1-14) and contains one FMN and eight iron-sulfur clusters. The Nqo3 subunit possesses fully conserved 11 Cys and 1 His in its N-terminal region and is considered to harbor three iron-sulfur clusters; however, only one binuclear (N1b) and one tetranuclear (N4) were previously identified. In this study, the ...

Oscillator strengths for the three lowest ground-state transitions 3p S0-3p 4s P1, 3p 6 S0-3p 4s P1, and 3p 6 S0-3p 3d P1 in argonlike K II are reported. The values were determined by lifetime measurements made using foil excitation of a fast ion beam, which yielded values t(4s P1)58.461.6 ns, t(3d P1)52.460.4 ns, and t(4s P1)50.4360.04 ns. Application of these results to the development of ext...

Many-body perturbation theory is implemented in order to calculate the isotope shifts of $4s$, $4p_{1/2}$, $4p_{3/2}$, $3d_{3/2}$, and $3d_{5/2}$ energy levels Ca$^+$, for even isotopes $A=$40, 42, 44, 46, 48. The results are presented mass shift field shift, as well higher-order shifts, quadratic nuclear polarization correction, cross term between shifts. Additionally, we examine King-plot non...

In this article, we study the blow-up and instability of standing waves for theinhomogeneous fractional Schrodinger equation $$ i\partial_tu-(-\Delta)^su+ |x|^{-b}|u|^{p}u=0, where \(s\in (\frac{1}{2},1)\), \(0<b<\min \{2s,N\}\) \(0<p< \frac{4s-2b}{N-2s}\). \(L^2\)-critical \(L^2\)-supercritical cases, i.e.,\(\frac{4s-2b}{N}\leq p< \frac{4s-2b}{N-2s}\), establish general criteria...

The distribtuion of various amino acid tRNA's in the 4S RNA components of avian myeloblastosis virus (AMV) and in 4S RNA prepared from chicken cmbryo cells, chicken myeloblasts, and chicken livers was determined. This was done by aminoacylating the 4S RNA samples with a mixture of 17 radioactive amino acids and subsequently identifying the tRNA-accepted amino acids on an amino acid analyzer aft...

Serumfree cultures of insect cells play an important role in the fields of protein engineering, medicine, and biology. In this paper, the suspension cell line QB-Tn9-4s of Trichoplusia ni (Hübner) (Lepidoptera: Noctuidae) was successfully adapted to serumfree Sf-900 III medium and passaged for 52 generations. The adapted QB-Tn9-4s cells grew faster. Their population doubling time shortened from...

The structure of activated pig heart aconitase [citrate(isocitrate) hydro-lyase, EC 4.2.1.3] containing a [4Fe-4S] cluster has been refined at 2.5-A resolution to a crystallographic residual of 18.2%. Comparison of this structure to the recently determined 2.1-A resolution structure of the inactive enzyme containing a [3Fe-4S] cluster, by difference Fourier analysis, shows that upon activation ...

We examine two-spin (2S) and cyclic four-spin (4S) magnetic interactions in insulating ladder and two-dimensional (2D) cuprates. By a comparison of eigenstates between d-p and Heisenberg models, we evaluate magnitudes of these interactions. We find that the 4S interaction is ∼10 % of nearest neighboring 2S interaction, and a diagonal 2S interaction is considerably small. The 4S interaction for ...