In the present study, three experiments were designed to identify most appropriate technique for freezing rabbit semen. Experiment 1 aimed determine optimal levels of dimethyl sulfoxide (DMSO) contents in medium and their effects on individual bucks. Semen ejaculates each buck (n=15 bucks) mixed split into portions extension with a containing varying concentrations DMSO (0.75, 1.0, 1.4 M). Dilu...

Previously, we have identified a membrane-mediated uptake and translocation of 1,3,5(10)-estratrien-3, 17beta-diol 6-(O-carboxymethyl)oxime:(125)I-labeled BSA (E6(125)I-BSA) in vivo in immature female rat liver from the plasma membrane (P3 fraction) to the mitochondria and/or lysosomes (P2 fraction). To further investigate this unique effect, current experiments have involved the use of 1,3,5(1...

D epartm ent of Radiology. University of California, San Francisco, California 94143, USA Z. Naturforsch. 41b, 1170—1177 (1986); received February 20, 1986 Bovine Serum Albumin, Gadolinium Complexes, Contrast Agents for NMR Imaging, Nitroxyls Bovine Serum Albumin (BSA) was spin labeled using the active esters 3 and 5 containing a nitroxyl radical to give the spin labeled BSA derivatives 4 and 6...

INTRODUCTION The livertaxis of glycyrrhizic acid-conjugated bovine serum albumin (GL-BSA) has been reported in the literature. Now, in this paper, we describe a novel type of drug-targeted delivery system containing 10-hydroxycamptothecin (HCPT) with liver tumor targeting. METHODS First, GL was coupled to BSA then HCPT was encapsulated in GL-BSA by high-pressure homogenization emulsification....

In this study, two following experiments were performed to improve post-thaw motility and viability of frozen-thawed ram spermatozoa. We examined i) the effects of different concentrations of bovine serum albumin (0, 0.3, 1, 5, 10 and 15% BSA) in semen diluents lacking egg yolk and ii) the effects of four semen diluents, fructose (F: control) and trehalose (T) in semen diluents containing egg y...

The present study was conducted to test the post-thaw sperm characteristics (motility, viability, acrosomal and plasma membranes integrity) through addition of different concentrations of BSA (0.50, 1.00, 5.00, 10.00 and15.00 %) to semen diluents lacking egg yolk. Semen was collected from four mature buffalo bulls, twice weekly using artificial vagina. Sperm characteristics (motility, viability...

kept at 29ºC for 10 days to allow the expression of the UAS constructs. Apc2 N175K is a loss-of-function allele, Apc Q8 is a null allele, UAS-Ras V12 and UAS-Tkv Q253 are gain-of-function transgenes. UAS-Tkv DN is a dominant negative transgene. Stocks were obtained form Bloomington Stock Center and VDRC. Staining and antibodies. Adult female flies were dissected in PBS. All the digestive tract ...

A potential new treatment for drug addiction is immunization with vaccines that induce antibodies that can abrogate the addictive effects of the drug of abuse. One of the challenges in the development of a vaccine against drugs of abuse is the availability of an optimum procedure that gives reproducible and high yielding hapten-protein conjugates. In this study, a heroin/morphine surrogate hapt...

OBJECTIVE To evaluate (1) to what extent sera from healthy subjects and patients with rheumatoid arthritis (RA) contain antibodies to bovine serum albumin (BSA); and (2) if anti-BSA antibodies interfere with results of enzyme-linked immunoassays (ELISA) containing BSA. METHODS The ELISA used was a previously developed in-house assay of autoantibodies to tumor necrosis factor (TNF). Anti-TNF a...

Adsorption of Bovine Serum Albumin (BSA) onto calcium Hydroxyl Apatite (HA), Ca10PO4(OH)2, has been studied by Ultra Violet (UV) spectroscopy. The adsorption isotherms of BSA onto the HA surface at 291.7 K and 303.2 K were satisfactorily presented by Langmuir equation and the evaluated parameters are reported. The specific surface area of H...