We report here a biophysical and biochemical approach to determine the differences in interactions of NiCR and NiCR-2H with DNA. Our goal is to determine whether such interactions are responsible for the recently observed differences in their cytotoxicity toward MCF-7 cancer cells. Viscosity measurement and fluorescence displacement titration indicated that both NiCR and NiCR-2H bind weakly to ...

Pentadecafluorooctanoic acid is an established peroxisome proliferator. Little is known about effects of treatment with 1H,1H,2H,2H-heptadecafluorodecan-1-ol, which is metabolized to pentadecafluorooctanoic acid. We compared effects of various dosages (3, 10, or 25 mg/kg body wt) of each of these compounds on hepatic gene expression in rats with microarrays. Microarray data were validated by re...

Both the Schiff base-mediated 2-keto-4-hydroxyglutarate aldolase of liver and the metal-activated a-keto-/3-deoxyhexarate aldolase of Escherichia coli condense pyruvate and glyoxylate to form (4-RS)-Z-keto-4-hydroxyglutarate. Both enzymes catalyzed a condensation between [3-3H3]pyruvate and glyoxylate. It was found that C-C synthesis occurred 6 to 7 times faster than C-T bond cleavage. Thus, py...

We study in detail locally Cohen-Macaulay curves in P 3 which are contained in a double plane 2H , thus completing the classification of curves lying on surfaces of degree two. We describe the irreducible components of the Hilbert schemes Hd,g(2H) of locally Cohen-Macaulay curves in 2H of degree d and arithmetic genus g, and we show that Hd,g(2H) is connected. We also discuss the Rao module of ...

utilization of photodisintegration reaction of deuterium, 2h(?,n)1h, is a precise technique for determining the percentage of 2h in tritiated and natural water. the 23na(n,?)24na reaction is a suitable ?-source for producing the 2h(?,n)1h reaction. tehran research reactor was used as a neutron source for producing 24na isotopes. the bf3 detectors were used to detect the neutron emission of h2 c...

A series of phthalimide derivatives planned as drugs candidates to treat the symptoms of sickle cell anemia were evaluated in a mutagenicity test using strains of Salmonella typhimurium TA100 and TA102, without and with addition of S9 mixture, with the aim to identify the best structural requirements for a drug candidate without genotoxic activity. The compounds (1,3-dioxo-1,3-dihydro-2H-isoind...

The single crystals of discrete hydrated [(OAc)Cu(mu-hep)(2)Cu(OAc)].2H(2)O (.2H(2)O) and [(OAc)Cu(mu-hep)(2) Cu(O(n)Pr)].2H(2)O (.2H(2)O) (the lattice H(2)O molecules exist as a tetrameric water cluster, hep-H = 2-(2-hydroxyethyl)pyridine), OAc(-) = acetate and O(n)Pr(-) = n-propionate) undergo single-crystal to single-crystal (SCSC) transformations to the dehydrated and , respectively, under ...

Several problems limit quantification of gluconeogenesis. We applied in vitro 2H-nuclear magnetic resonance (NMR) spectroscopy to simultaneously measure 2H in all glucose carbons for direct assessment of gluconeogenesis. This method was compared with 2H measurement in carbons 5 and 2 using gas chromatography-mass spectrometry (hexamethylenetetramine [HMT]) and with in vivo 13C magnetic resonanc...

The article argues that the EPP should be eliminated. It is shown that in a number of constructions the EPP does not hold at all. Where it does appear to hold, its effects follow from independent mechanisms of the grammar. EPP effects concerning the final landing site of A-movement follow from Case theory. Intermediate [Spec,IP]s are filled as a result of the requirement of successive cyclicity...