Two-dimensional gel electrophoresis (2-DE) methods such as two-dimensional polyacrylamide gel electrophoresis (2D-PAGE; O’Farrell (1975)) and two-dimensional difference gel electrophoresis (2D-DIGE; Ünlü et al. (1997)) are popular techniques for protein separation because they allow researchers to characterize quantitative protein changes on a large scale. Thus, 2-DE is frequently used as an in...

BACKGROUND Resveratrol, a phytochemical present in grapes, berries, and red wines, has been reported to induce apoptosis in various cancer cells. To explore the molecular mechanisms involved in the anticancer activity, the apoptotic activity of resveratrol in hepatic cancer cells was investigated. MATERIALS AND METHODS 1-Dimensional (1D) and 2-dimensional (2D) polyacrylamide gel electrophores...

The technique of two-dimensional (2D) gel electrophoresis is a powerful tool for separating complex mixtures of proteins, but since its inception in the mid 1970s, it acquired the stigma of being a very difficult application to master and was generally used to its best effect by experts. The introduction of commercially available immobilized pH gradients in the early 1990s provided enhanced rep...

A novel elastic image registration approach is proposed, which can register both 2D medical images and gel electrophoresis automatically and accurately. Firstly, the global affine registration method based on image intensity is used to initialise a starting estimation of the robust registration algorithm. Then, by using iterative landmark based algorithm, the affine registration results are reg...

conclusions our successful extraction of purified lps from e. coli isolates of uti patients’ urine samples can be an important step to understand the uti disease conditions. results the silver-stained gel demonstrated both smooth and rough type lps by showing trail-like band patterns with the presence and lacking o-antigen region, respectively. coomassie blue staining showed no band assuring th...

Presentations at the 2008 meeting of the American Electrophoresis Society dealt with many aspects of this key separation technology. In total, there were 65 technical talks and 13 posters in a 4-day meeting. The proteomics technical talks described applications of 1D and 2D gel electrophoresis, capillary electrophoresis and microscale platforms. Some new platforms may find use in future proteom...

These studies were structured with the aim of utilizing emerging technologies in two-dimensional (2D) gel electrophoresis and mass spectrometry to evaluate protein expression changes associated with type 1 diabetes. We reasoned that a broad examination of diabetic tissues at the protein level might open up novel avenues of investigation of the metabolic and signaling pathways that are adversely...

UNLABELLED Image analysis of two-dimensional gel electrophoresis is a key step in proteomic workflow for identifying proteins that change under different experimental conditions. Since there are usually large amount of proteins and variations shown in the gel images, the use of software for analysis of 2D gel images is inevitable. We developed open-source software with graphical user interface ...