Two human papillomavirus (HPV) 16 oncogenic proteins, E6 and E7, are co-expressed in the majority of HPV16-induced cervical cancer cells. Thus, the E6 and E7 proteins are good targets for developing therapeutic vaccines for cervical cancer. In the present study, immunization with the mutant non-transforming HPV16 E7 (mE7) protein was demonstrated to inhibit the growth of TC-1 cells in the TC-1 ...

Hepatitis C virus (HCV) infection in hepatocytes stimulates innate antiviral responses including the production of type III interferons (IFN-λ), including IL-28A, IL-28B, and IL-29. However, the molecular mechanism(s) regulating the expression of IFN-λ genes in HCV-infected hepatocytes remains undefined. In this study, we examined regulatory elements involved in the induction of IFN-λ genes fol...

Hepatitis C virus (HCV) infection in hepatocytes stimulates innate antiviral responses including the production of type III interferons (IFN-l), including IL-28A, IL28B, and IL-29 (Figure 1). However, the molecular mechanism(s) regulating the expression of IFN-l genes in HCV-infected hepatocytes remains undefined. In this study, we examined regulatory elements involved in the induction of IFN-l...

Hydrogen peroxide (H2O2) is an important signaling molecule that involved in multiple physiological metabolic processes plants. Excess H2O2 can destroy biological macromolecules to poison the cell. Thioredoxin peroxidase (Tpx) plays role protecting plants from oxidative damage by clearing H2O2. In this study, tomato Tpx (SlTpx) gene was cloned and bioinformatic analysis done. The mRNA transcrip...

introduction: neisseria meningitidis is a major causative agent of bacterial septicemia and meningitis in human. pora is a major component of the outer membrane of n. meningitidis and functions as a cationic porin. this study aimed to clone and determine the expression of pora as the first step for producing a proper antigen in a vaccine study against n. meningitidis. methods: an approximately ...

Activation of host innate immune responses was studied in severe acute respiratory syndrome coronavirus (SCV)-infected human A549 lung epithelial cells, macrophages, and dendritic cells (DCs). In all cell types, SCV-specific subgenomic mRNAs were seen, whereas no expression of SCV proteins was found. No induction of cytokine genes (alpha interferon [IFN-alpha], IFN-beta, interleukin-28A/B [IL-2...

Three isostructural interwoven 3,4-connected mesoporous metal-organic frameworks of pto-a topology (UTSA-28-Cu, UTSA-28-Zn, and UTSA-28-Mn) were synthesized and structurally characterized. Because of their metastable nature, their gas sorption properties are highly dependent on the metal ions and activation profiles. The most stable, UTSA-28a-Cu, exhibits promising gas storage and separation ca...

The current standard of care for the treatment of chronic hepatitis C is pegylated interferon-α (PEG-IFNα) and ribavirin (RBV). The treatment achieves a sustained viral clearance in only approximately 50% of patients. Recent whole genome association studies revealed that single nucleotide polymorphisms (SNPs) around IL-28B have been associated with response to the standard therapy and could pre...

Human rhinovirus (HRV) infections are associated with the common cold, occasionally with more serious lower respiratory tract illnesses, and frequently with asthma exacerbations. The clinical features of HRV infection and its association with asthma exacerbation suggest that some HRV disease results from virus-induced host immune responses to infection. To study the HRV-infection-induced host r...

Long-chain scorpion toxins with four disulfide bridges exhibit various pharmacological features towards the different voltage-gated sodium channel subtypes. However, the toxin production still remains a huge challenge. Here, we reported the effects of different expression vectors on the pharmacological properties of a novel toxin BmαTX47 from the scorpion Buthus martensii Karsch. The recombinan...