The diversity of endosymbiotic dinoflagellates (Symbiodinium) in corals at Johnston Atoll in the central Pacific Ocean was assessed using both the internal transcribed spacer 2 (ITS2) region of the nuclear rDNA and chloroplast 23S rDNA. More sequences were recovered from corals using the ITS2 primers than with the chloroplast 23S primers, a finding that reflects both the higher taxonomic resolu...

Psyllids are plant sap-feeding insects that harbor prokaryotic endosymbionts in specialized cells within the body cavity. Four-kilobase DNA fragments containing 16S and 23S ribosomal DNA (rDNA) were amplified from the primary (P) endosymbiont of 32 species of psyllids representing three psyllid families and eight subfamilies. In addition, 0.54-kb fragments of the psyllid nuclear gene wingless w...

In an attempt to clarify the taxonomy of the Mycobacterium avium complex, the relationship between IS1245 RFLP, growth temperature, 16S rDNA signature sequences and the 16S-23S rDNA internally transcribed spacer (ITS) of 160 M. avium-complex isolates from different sources was investigated. All 70 isolates identified as M. avium by INNO-LiPA MYCOBACTERIA (Innogenetics, Belgium), a DNA probe tes...

Objective(s) In addition to several molecular methods and in particular 16S rDNA analysis, the application of a more discriminatory genetic marker, i.e., 16S-23S internal transcribed spacer gene sequence has had a great impact on identification and classification of mycobacteria. In the current study we aimed to apply this sequencing power to conclusive identification of some Iranian clinical ...

Annotation of rRNA genes has been incomplete in Agrobacterium species although a number of Agrobacterial rDNA fragments have been sequenced. In this study, precise characterization of rRNA operons (rrn) was carried out in two biovar 1 strains, C58 and MAFF301001. Complete DNA sequencing of four rrns in MAFF301001 indicated that each operon codes for 16S, 23S and 5S rRNA as well as three tRNAs, ...

The nucleotide sequences of the rRNA genes and the 5' flanking region were determined for R. salmoninarumATCC 33209T from overlapping products generated by PCR amplification from the genomic DNA. Comparisonof the sequences with rRNA genes from a variety of bacteria demonstrated the close relatedness between R.salmoninarum and the high G+C group of the actinobacteria, in particul...

Leptospirosis, a disease with protean manifestations, is caused by Leptospira species complex. A definite diagnosis of leptospirosis during the first week of the illness is a major challenge to investigating clinical laboratories. PCR is a rapid, sensitive and specific means of diagnosing Leptospira infections in the early phase of the disease. In this study, the applicability of routine PCR as...

The genus Carnobacterium is currently divided into the following eight species: Carnobacterium piscicola, C. divergens, C. gallinarum, C. mobile, C. funditum, C. alterfunditum, C. inhibens, and C. viridans. An identification tool for the rapid differentiation of these eight Carnobacterium species was developed, based on the 16S-23S ribosomal DNA (rDNA) intergenic spacer region (ISR). PCR-restri...

AIMS Fourier transform infrared (FT-IR) was used to analyse a selection of Acinetobacter isolates in order to determine if this approach could discriminate readily between the known genomic species of this genus and environmental isolates from activated sludge. METHODS AND RESULTS FT-IR spectroscopy is a rapid whole-organism fingerprinting method, typically taking only 10 s per sample, and ge...