Aflatoxin B2a (AFB2a) antiserum has been previously used in an enzyme-linked immunosorbent assay (ELISA) for the quantitation of AFB1 and AFB2a. The present investigation examined the reactivity of the antiserum toward those adducts and metabolites of AFB1 believed to play a major role in aflatoxicosis and carcinogenesis. 2,3-Dihydro-2-(N7-guanyl)-3-hydroxyaflatoxin B1 (AFB1-N7-Gua), the putati...

Male Fisher 344 rats were fed diets containing either 20% corn oil, 20% coconut oil or 18% coconut oil plus 2% corn oil for 3 wk. A single dose of [3H]aflatoxin B1 [(3H]AFB1) was administered ip and the biliary excretion of aflatoxin metabolites and the binding of 3H to nucleic acids were studied. In other experiments the in vitro metabolism of AFB1 by liver postmitochondrial supernatants prepa...

This study aimed to prepare the basic components of aflatoxin B1 (AfB1) radioimmunoassay (RIA) technique using liquid phase to measureAfB1 in agricultural commodities. These components were polyclonal antibody (anti-AfB1), I-labelledAfB1 and aflatoxin B1 standards.Anti-AfB1 polyclonal was raised by immunizing six white New-Zealand rabbits with aflatoxin B1bovine serum albumin (AfB1-BSA) conjuga...

the inhibitory activities of aqueous and solvent extracts of twelve selected medicinal plants were evaluated against biosynthesis of aflatoxin b1 (afb1) by aspergillus flavus. the a. flavus was isolated from maize, and aflatoxin b1 biosynthesis was confirmed by comparison with standard afb1 using tlc method. in vivo antiaflatoxigenic efficacies of activity guided solvent extracts were determine...

Crops contaminated with fungal mycotoxins such as aflatoxin B1 (AFB1) are often downgraded or removed from the food chain. This study aimed to evaluate the tolerance and accumulation of AFB1 in two insect species to determine whether they could be used to retain condemned mycotoxin contaminated crops in the food chain. First, instar black soldier fly larvae (Hermetia illucens, BSF) and yellow m...

introduction: aflatoxin b1 (afb1) is one of the most well-known hepatocarcinogens in humans. contamination of raw materials, used in the production of sausages and burgers, with aflatoxin producing molds can lead to increased level of aflatoxin in the final products and can impose hazards to human health. unfortunately, aflatoxin is resistant to heating and freezing processes, etc. and can rema...

Aflatoxin B1 (AFB1) and its metabolite aflatoxin M1 (AFM1) are well-known carcinogens for humans and animals health. In this study, an ultra-high performance liquid chromatography linked with fluorescence detection (UPLC-FLD) method was optimized and validated. In addition, we investigated for the first time, the influence of curcumin on residue depletion of AFB1 and AFM1 in liver, kidney, and ...

Mycotoxins are harmful auxiliary metabolites delivered by species of filamentous organisms developing on grains some time recently collect and in capacity. The study was planned to evaluate the protective role lycopene (LYC) against Aflatoxin B1 (AFB1) induces oxidative stress, hepatic toxicity, apoptosis Japanese quail. Sixty quail chicks (three-week-old) were randomly allocated into four grou...

Biosynthesis of the highly toxic and carcinogenic aflatoxins in select Aspergillus species from the common intermediate O-methylsterigmatocystin has been postulated to require only the cytochrome P450 monooxygenase, OrdA (AflQ). We now provide evidence that the aryl alcohol dehydrogenase NorA (AflE) encoded by the aflatoxin biosynthetic gene cluster in Aspergillus flavus affects the accumulatio...

A total of 540 one-day old male broilers Ross 308 were allocated to 6 treatments in a completely randomized designed with a 2×3 factorial arrangement (aflatoxin B1 (AFB1) and probiotics) with 6 replicates and 15 birds in each. Factors include aflatoxin B1 (control, 500 ppb) and probiotics (control, 108 cfu/mL Bacillus sp. MBIA2.40, 0.2 g Gallipro per kg). Results showed that adding 500 ppb AFB1...