Two new Uromunna species, mundongensis sp. n. and jejuensis n., are described from the Korean Peninsula, representing first record of genus in waters. Genetic analyses using two mitochondrial (COI, Cytb) one ribosomal (18S rRNA) gene allowed us to confirm high pair-wise interspecific differences with establishment reliable barcoding gaps COI (19.9%) 18S (0.4%) between species. Finally, phylogen...

Identifying suitable housekeeping genes for quantitative RT-PCR in the uterus is problematic, as this tissue undergoes significant structural and functional alterations during the oestrous cycle and pregnancy in response to circulating hormones. The suitability of 18S rRNA as a housekeeping gene in mouse uterus was investigated by introducing an 'RNA spike' standard into the reverse transcripti...

The 18S rRNA gene is fundamental to cellular and organismal protein synthesis and because of its stable persistence through generations it is also used in phylogenetic analysis among taxa. Sequence variation in this gene within a single species is rare, but it has been observed in few metazoan organisms. More frequently it has mostly been reported in the non-transcribed spacer region. Here, we ...

Theileria parva is the causative agent of Corridor disease in cattle in South Africa. The African buffalo (Syncerus caffer) is the reservoir host, and, as these animals are important for eco-tourism in South Africa, it is compulsory to test and certify them disease free prior to translocation. A T. parva-specific real-time polymerase chain reaction (PCR) test based on the small subunit ribosoma...

sarcocyst is one of the most important protozoa belonged to apicomplexa. this parasite is prevalent among warm blooded animals throughout the world. in the present work, sarcocystis gigantea was identified by amplification of 18s rrna gene using pcr- rflp. in this regard macroscopic cysts of sarcocystis were collected from esophagus and intra costal muscles of sheep in shahriar slaughterhouse. ...

considering the importance of theileria equi infection in horse breeding industry and marketing, in the present study, we aimed to determine the prevalence of t. equi among dromedaries in central iran, where a considerable number of camels and horses are raised and equine theileriosis is quite prevalent. for this purpose, a total of 161 blood samples from camels were examined in terms of t. equ...

BACKGROUND The appropriate choice of an internal reference is critical for quantitative RNA analysis. However, no comparison of frequently used "housekeeping" genes is available for renal biopsy studies. METHODS Microdissected biopsies from 165 patients, including a wide range of histopathologic diagnoses, were analyzed [immunoglobulin A (IgA) nephritis, membranous glomerulopathy, rapid progr...

Quantitative RT-PCR analysis. The following primers were used to amplify β-casein cDNA and 18S rRNA sequences: forward primer of the β-casein gene 5'-GCT CAG GCT CAA ACC ATC TC-3' and reverse primer 5'-TGT GGA AGG AAG GGT GCT AC-3'; forward primer of the 18S rRNA gene 5’-TCGGAACTGAGGCCATGATT-3’ and reverse primer 5’CCTCCGACTTTCGTTCTTGATT-3’. Fragments of β-casein gene and 18S rRNA were amplifie...

The present experiment is an effort to find a stable reference gene in Camellia sinensis and Camellia assamica under different biotic and abiotic stresses. This study evaluate the variation in gene expression across tea leaf tissues in nine experiments. The suitability of 18S rRNA, 26S rRNA, rubiscobis-phosphatase oxygenase (RuBP) and Camellia tubulin (CaT) as reference genes were validated by ...

The prevalence of Hepatozoon parasites in 460 lizards from North Africa was studied by amplification and sequencing of the 18S rRNA gene. The phylogenetic analysis of the 18S rRNA gene provides new insights into the phylogeny of these parasites with multiple genetically distinct lineages recovered. Parasite prevalence differed significantly between lacertid lizards and geckos. Our results show ...