Discrimination of Staphylococcus aureus strains, isolated from camel abscesses and mastitic milk of camel, cattle and goats, on the basis of 16S-23S ribosomal RNA intergenic space polymorphism was carried out. Two sets of primers were used for amplification of DNA of intergenic space; the one having a highly conserved sequence in eubacterial 23S rRNA transcript, while the other having less cons...

Urtica dioica is an important medicinal plant which is widely distributed in Mazandaran province (North of Iran). In this study for the first time Amplified Fragment Length Polymorphism (AFLP) and Inter-simple Sequence Repeat (ISSR) markers were used for detection of genetic polymorphism in Mazandaran nettle. Ten AFLP primer combinations and seventeen ISSR markers were utilized. AFLP produced 830...

The degree of genetic diversity in 45 Bordetella (B.) bronchiseptica strains comprised of a vaccine strain (N = 1), reference strains (N = 3) and field isolates (N = 41) was evaluated using random amplified polymorphic DNA (RAPD) fingerprinting and pulsed-field gel electrophoresis (PFGE). Three candidate primers were selected for RAPD analysis after screening 20 random decamer oligonucleotides ...

genetic diversity relationships of 50 isolates of cytospora schulzeri on apple from different parts of the semirom region were analyzed using 15 polymerase chain reaction (pcr) based markers, 7 random amplified polymorphic dnas (rapds) and 8 microsatellite primed polymerase chain reaction (mp-pcr). using 7 selected rapd primers 113 bands were generated, of which 81 bands were polymorphic (71.7%...

The genetic diversity of Valsa sordida isolates from different geographical regions and hosts was investigated using MP-PCR markers. PCR amplifications were done eight primers. Of them, only four primers [(ATC)7, (ACTG)4, (CGA)5, (AAC)8] produced polymorphic bands. At least 88.5% polymorphism revealed by the maximum (97%) generated (ACTG)4 primer three diagnosable groups (1, 2 3) resolved in re...

The present study was conducted to investigate genetic diversity between and within of six populations with different individual numbers of cultivated and non-cultivated provenances of  Moringa peregrina (Forssk.) Fiori and Moringa oleifera Lam. using RAPD, ISSR and various combinations of RAPD and ISSR primers in one multiplex PCR (R-ISSR). 10 primers that produced clear and ...

This study was accomplished to detect variation generated through eposure of fenugreek (trigonella foenum-graecum L.) seeds seven gamma irradiation doses (0,25,50,100,150,300 and 400) GY by using twenty RAPDs primers as molecular marker ,results indicated that OPA-14 ,OPC-19 OPH-01success in giving five treatments a unique fingerprint. Molecular size amplicon ranged between 2913 bp produced pri...

This study describes the genetic relationships among 34 varieties of Lallemantia iberica using inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP). Samples were collected from Agriculture Research Center of Urmia city (northwest Iran). Ten IRAP and REMAP primers generated 76 scorable electrophoretic bands with 78.94% pol...

Free-living N2-fixing Frankia strains isolated from Casuarina sp. were investigated for genomic polymorphism. We used six 10-mer oligonucleotides as single arbitrary primers (AP) for the polymerase chain reaction (PCR) in order to amplify random DNA fragments in the genome of free-living Frankia strains. Agarose-gels of the amplified genomic DNA revealed that two of the six arbitrary primers sh...