abstract as hybrid spawn is propagated asexually in edible mushrooms, producers may propagate it without the permission of the owner. for the protection of the owner right, there is a great need for a molecular certificate for each commercial line. the high-resolution genotyping method of amplified fragment length polymorphism (aflp) analysis was used to prepare molecular certifications and fin...

Sampling was done using 90 post larvae which were produced by reproduction of some broodstocks of Fenneropenaeus indicus in one day and reared in the same situation for 4 months. Samples were divided into 3 groups: high, medium and low growth (based on weight and length). Genomic DNA was extracted from muscle tissue using the phenol-chloroform method. The polymerase chain reaction (PCR) was car...

to evaluate the genetic variations in eleven fennel populations in iran, 55 accessions were analyzed by srap markers. twelve primer combinations produced 171 polymorphic bands. the cumulative dendrogram delineated fennel accessions into five major groups in accordance to the geographical regions from which the populations were originated. about 43.57% of total genetic variation was detected amo...

Sampling was done using 90 post larvae which were produced by reproduction of some broodstocks of Fenneropenaeus indicus in one day and reared in the same situation for 4 months. Samples were divided into 3 groups: high, medium and low growth (based on weight and length). Genomic DNA was extracted from muscle tissue using the phenol-chloroform method. The polymerase chain reaction (PCR) was car...

genetic diversity of 36 genotypes of dwarf and semi-dwarf apple rootstocks along with seven iranian dwarf rootstocks were investigated by use of rapd dna markers. a set of 100 random primers was utilized in pcr reaction from which 10 primers resulted in good amplification with high polymorphism. among primers, 10 selected rapd primers produced 160 bands, in which, 136 were polymorphic. cluster ...

west part of iran is considered as one of the center of diversity for aegilops triunsialis, therefore, study of its genetic diversity in this area is extremely important regarding evolution of this plant. the genetic variation among 40 accessions of ae.triunsialis collected from west of iran were studied using 12 issr markers. dna was extracted, concentrations adjusted and five samples from eac...

The morphological characters of Cirrochroa aoris (Large Yeoman) and C. tyche (Common Yeoman) are very similar making identification confusing and difficult. The genomic DNA of the two species was subjected to RAPD-PCR analysis with six decamer oligonucleotides, i.e. MA5, MA6, MA8, OPB12, OPT4 and OPT5. All of them produced discrete bands of various lengths revealing genetic variations as well a...

DNA from 14 Pythium species, Verrucalvus flavofaciens and Zoophagus insidians was characterized by CsCl-bisbenzimide density gradients in order to investigate its taxonomic potential. A few incomplete analyses were made for other species. All clearly assignable Pythium species produced three DNA bands in the gradient. Pythium undulatum along with Verrucalvus and Zoophagus produced only two band...

pomegranate is an importantly popular fruit crop with iran the main center of its diversity throghout the world. the richest pomegranate genetic resources believed to exist in iran. the present investigation was carried out to study the genetic diversity of 15 genotypes of pomegranate in tarume zanjan making use of rapd markers. genomic dna was extracted from the plant,s leaf, and tested for th...