Yersinia enterocolitica is an important pathogen to animals and humans. Thirteen strains of Y. enterocolitica were isolated from diseased fish. The partial 16SrDNA gene, five virulence genes, pathogenicity, and drug resistance of Y. enterocolitica strain G6029 were studied using molecular biological technology and toxicological method. Results showed that the length of amplified 16SrDNA sequenc...

aims: it is very important to detect the coagulase-negative staphylococci, which produce the hospital infections. being one of the most expensive and time-consuming stages before the polymerase chain reaction (pcr), dna extraction is one of the primary stages of pcr. then, it should be noticed that the elimination of the stage might save time and costs. the aim of this study was to compare two ...

Article history: Received on: 25/04/2016 Revised on: 11/05/2016 Accepted on: 08/06/2016 Available online: 30/08/2016 Strain VE2 was isolated from the stem tissue of Vernonia cinerea (L.) Less. and identified as Streptomyces sp. on the basis of morphology, chemotaxonomy and 16SrDNA sequencing. The fractionation of the crude ethyl acetate (CEA) extract from VE2 cultures led to the isolation of tw...

A gene fusion encoding a plastid-targeted bacteriophage T7 RNA polymerase (T7RNAP) under the transcriptional control of the light-regulated promoter and the plastid-targeting signals of a ribulose-bisphosphate carboxylase/oxygenase (Rubisco) small-subunit (SSU) gene was introduced into the nuclear genome of Nicotiana tabacum (tobacco). Immunoblot analysis, in vitro transcription assays and prot...

Sixteen bacterial strains showing oval, convex with a white colony colour were obtained from the water-soaked lesions on petioles and leaves of infected papaya (cv. calina) collected field in Lampung Timur, Indonesia. The pathogenicity test showed that all produced same symptoms those found field. Four representative then chosen for further investigation. phenotypic characteristics revealed res...