objective(s): the development of reliable and ecofriendly process for the synthesis of nano-metals is an important aspect in the field of nanotechnology. nano-metals are a special group of materials with broad area of applications. materials and methods: in this study, extracellular synthesis of silver nanoparticles (snps) performed by use of the gram positive soil streptomycetes. streptomycete...

in this research the effects of crude oil on morphological and physiological characterization ofthe cyanobacterium microchaete tenera isc13 were investigated. isolated cyanobacterium treated with different oil concentrations (control, 1, 2.5, 5 and 7%) in carbonless bg110 medium. morphological characteristics such as morphology of filament, cellular shapes and sizes, relative position of hetero...

background and objectives: the development of molecular biological techniques provide a method for direct detection and identification of pathogens. this study attempted to compare bacterial culture and pcr to detect s. agalactiae in cattle milk samples. the pcr method was based on using primers v1 and v2 derived from the 16s rrna gene. materials and methods: one hundred milk samples were colle...

objective(s) in addition to several molecular methods and in particular 16s rdna analysis, the application of a more discriminatory genetic marker, i.e., 16s-23s internal transcribed spacer gene sequence has had a great impact on identification and classification of mycobacteria. in the current study we aimed to apply this sequencing power to conclusive identification of some iranian clinical s...

uricase or urate oxidase (urate:oxygen oxidoreductase, ec 1.7.3.3), a peroxisomal enzyme which is found in many bacteria, catalyzes the oxidative opening of the purine ring of urate to yield allantoin, carbon dioxide, and hydrogen peroxide. in this study, the phylogeny of urate oxidase (uricase) producing bacteria was studied based on gene sequences of 16s rrna and uricase protein. representati...

The influence of parameter changes on the bacterial community of a laboratory-scale anaerobic digester fed with glucose was investigated using a culture-independent approach based on single-strand conformation polymorphism (SSCP) analysis of total 16S rDNA and 16S rRNA amplification products. With the digester operating at steady state, the 16S rDNA SSCP patterns of the bacterial community show...

We developed a novel method for the quantitative detection of the 16S rRNA of a specific bacterial species in the microbial community by using deoxyribozyme (DNAzyme), which possesses the catalytic function to cleave RNA in a sequence-specific manner. A mixture of heterogeneous 16S rRNA containing the target 16S rRNA was incubated with a species-specific DNAzyme. The cleaved target 16S rRNA was...

Nucleic acid sequences of Mycobacterium leprae were detected using gene probes hybridizing with targeting ribosomal RNA (16S rRNA), ribosomal DNA (16S rDNA) and gene amplification techniques (PCR) in skin lesion of paediatric leprosy patients and the effect of treatment on the by these methods. Eighty paediatric leprosy patients were included in the study. Most cases (79%) were between 9 and 16...