in the present study, an efficient protocol has been developed for callus induction and production of ra in callus culture of satureja khuzistanica for the first time. in vitro callus induction was achieved from young shoot tip explants cultured on ms and b5 media supplemented with different concentrations of iba (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins bap and kin...

In vitro multiple shoot regeneration of Solanum nigrum L., an Indian medicinal plant was accomplished on MS medium utilizing shoot tip and nodal explants. Direct multiple shoots differentiated within 6 weeks when explants were cultured on MS medium containing BAP (1.0-5.0 mg/l) and KIN (1.0-5.0 mg/l) individually. Among various concentrations of cytokinins tested, maximum number of multiple sho...

sugar type and growth regulators are essential fundamental components of tissue culture media. in this study, different concentrations of some carbohydrate and growth regulators during anther culture and producing somatic embryo of datura stramonium l. was investigated in ms medium. the ms medium supplemented with 0.5 mgl-1 kinetin and 2 mgl-1 2,4-d, and also the medium supplemented with 0.25 m...

tissue culture is an attractive alternative for plant propagation. micropropagation is a technique to ensure a constant and uniform source of ornamental plants. matthiola incana is an important ornamental species mainly cultivate by seed. matthiola incana seeds were germinated on solid ms medium without plant growth regulators. shoot proliferation and root formation are possible using kinetin (...

lisiantus is introduced among the 10 top cutting flowers in the world. micropropagation can be used in this plant because of some problems in traditional propagation. in this research, the effects of the growth regulators on the in vitro shoot regeneration and rooting of the shoots obtained from nodal explants were investigated. the effects of the different concentrations of bap and kin on shoo...

Tissue culture is an attractive alternative for plant propagation. Micropropagation is a technique to ensure a constant and uniform source of ornamental plants. Matthiola incana is an important ornamental species mainly cultivate by seed. Matthiola incana seeds were germinated on solid MS medium without plant growth regulators. Shoot proliferation and root formation are possible using kinetin (...

Transverse thin cell layer sections excised from in vitro scales of Lilium ledebourii were cultured on Murashige and Skoog (MS) medium supplemented with various plant growth regulators (PGRs) at different concentrations. Although, bulblets were produced on PGRfree MS medium during organogenesis, addition of 0.25 mg l-1 6-benzyladenine or 5.0 mg l-1 indole-3-acetic acid to the medium increased t...

Objective: In vitro propagation of AZ x M9 new apple rootstock resulted of breeding program of vegetative apple rootstock in Iran was investigated. Methods: Nodal explants were inoculated in Murashige and SKoog (MS) medium supplemented with various concentrations of 6-benzylaminon purine (BAP) (0.4, 0.8 and 1.2 mg/l) alone or with naphthalene acetic acid (NAA) (0.1 mg/l). We examined t...