DNA based RAPD (Randomly Amplification of Polymorphic DNA) markers have been used extensively to study genetic relationships in number of crop plants. In this study, 44 okra genotypes collected from different parts of India, were selected to assess genetic distinctiveness and relatedness. Total genomic DNA was extracted and subjected to RAPD analysis using 14 arbitrary 10 mer primers. The molec...

The availability of adequate information about the documentation and characterization germplasm is fundamental for any crop improvement program. importance cumin as a medicinal plant yet lack its genetic variability encouraged us to initiate current study aiming at assessing among 17 genotypes from different geographical regions using four molecular markers (RAPD, ISSR, SRAP SCoT). Further, pot...

Genetic diversity among ginger genotypes collected from different parts of the country was studied using molecular markers (30 RAPD and 55 SSR). Compared to primers SSR were efficient in distinguishing genotypes. A total 86 23 polymorphic bands observed with primers, respectively. Percentage polymorphism between 97.40 % 56.54 %. Grouping by combined data indicated that irrespective their place ...

The genetic distance of 11 cotton genotypes varying in heat tolerance was studied using RAPD markers. Fifty-three random decamer primers were used for the estimation of genetic distance. Among the 53 RAPD primers, which were custom synthesized by GeneLink Inc., UK, 32 were polymorphic and 21 were monomorphic. The 32 polymorphic primers produced 273 fragments, with a mean of 8.3 fragments per pr...

introduction despite the fact that iran is one of the major chickpea (cicer arietinum l.) center of origins, limited information is available regarding chickpea genetic variation and diversity. genetic diversity information is crucial for the choice of proper parents to establish new breeding programs. chickpea germplasm is poor, suggesting the need for gaining enough knowledge of genetic diver...

Random ampli®ed polymorphic DNAs (RAPDs) were used to discriminate among 25 Feijoa sellowiana cultivars and accessions. Fifty ten-mer oligonucleotide primers were tested on ®ve accessions. Twenty-two primers, showing clear polymorphic patterns, were chosen to amplify the DNAs of all 25 genotypes; each ampli®cation was repeated three times separately. Only 11 primers were ®nally analysed, yieldi...

Sudden death syndrome of soybean, caused by Fusarium virguliforme, is one of the most damaging diseases affecting soybean production in the Midwestern region of the USA. To date, there has been very low genetic variation detected in F. virguliforme populations, although isolates can exhibit differing aggressiveness on soybean. To further investigate the genetics behind this variation in aggress...

Dalbergia oliveri is a leguminous tree of the Fabaceae family. This species is popular and valuable in Vietnam and is currently listed on the Vietnam Red List and on the IUCN Red List as endangered. Two PCR techniques using RAPD and inter-simple sequence repeat (ISSR) markers were used to make a comparative analysis of genetic diversity in this species. Fifty-six polymorphic primers (29 R...