In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In-vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgL(-1)) solely or in combination with cytokinins BAP and K...

Apple is one of the most important temperate-zone fruit trees in the world. Micropropagation of apple scion and rootstocks is an important method of rapid production of healthy and disease-free plants. In this research, in vitro propagation of three apple roottocks including Azayesh-Esfahan, Morabbaee-Mashhad and M9 was investigated. Shoot proliferation carried out in two basal media (MS and WP...

  The acute toxicity and effects of diazinon on some haematological parameters of kutum (Rutilus frisii kutum, Kamensky, 1901) weighing 613.33 ± 157.06 g were studied under static water quality conditions at 15 °C ± 2 ºC. These experiments were carried out based on the standard TRC, 1984 method over 4 days, and controlled for the effective water physicochemical factors having pH rangesof 7-8.2,...

In vitro clonal multiplication of apple rootstock MM 111 using axillary buds and shoot apices were carried out. Vegetative axillary buds of the size of 0.2-2.0 cm and shoot apices measuring 4 mm in length were initiated to shoot proliferation on MS medium supplemented with BA (0.5 - 1.0 mgl(-1)), GA3(0.5 mgl(-1)), with or without IBA(0.05 - 0.1 mgl(-1)). Small size explants showed less phenol e...

Pyridine, a suspected genotoxic and formaldehyde, a potent carcinogen, are present in several industrial wastewater including commercial solvent, resin and pesticide manufacturing industries. The extent of pyridine and formaldehyde toxicity in aquatic organisms is unknown. Therefore, in present study the toxicity of pyridine and formaldehyde were investigated in artificial aquatic ecosystem (mi...

Pistachio is one of the most important nuts and its mass production using tissue culture techniques is of great importance. Micro-propagation has many complications. Contamination is one of the critical limitations in the establishment stage. This study focused on different NaClO and HgCl2 concentrations and exposure times. Shoot tip necrosis (STN) is one of the most common problems during larg...

In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins BAP and KIN...

thyme (thymus vulgaris l.) is an aromatic and medicinal plant which is very important for the herbal industry. in this research, in vitro selection for nacl tolerance was investigated in this plant. hypocotyl and shoot explants (apical meristem and cotyledonary leaves) of sterilized seedlings were cultured in ms medium supplemented with different combinations of 2, 4-dichlorophenoxyacetic acid ...

In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins BAP and KIN...