A loss of potency as one ascends a homologous series of compounds (cutoff effect) is often used to map the dimensions of binding sites on a protein target. The implicit assumption of steric hindrance is rarely confirmed with direct binding measurements, yet other mechanisms for cutoff exist. We studied the binding and effect of a series of n-alkanols up to hexadecanol (C16) on two model protein...

The OCT plasmid encodes enzymes for alkane hydroxylation and alkanol dehydrogenation. Structural components are encoded on the 7.5-kilobase pair alkBAC operon, whereas positive regulatory components are encoded by alkR. We have constructed plasmids containing fusions of cloned alkBAC and alkR DNA and used these fusion plasmids to study the functional expression of the alkBAC operon and the regu...

To characterize the cellular stress by n-alkanols with different alkyl chain lengths in Escherichia coli, we investigated how damage cell envelope permeability and whether they enhance promoter activity of response regulator, σE, using variants green fluorescent protein (GFP). By E. coli cells having GFPuv expressing localizing cytoplasm, inner membrane, periplasm, after exposure to n-alkanols,...

Mesoporous silica has been used as a heterogeneous support for catalysts; however, asymmetric induction by the helicity of inorganic mesoporous silica itself has not yet been achieved. P- and M-helical mesoporous silica was found to act as a chiral inorganic trigger for asymmetric autocatalysis to afford (S) and (R)-pyrimidyl alkanol with >99.5% ee, respectively.

In this work, cuticular waxes from flag leaf blades and peduncles of Triticum aestivum cv. Bethlehem were investigated in search for novel wax compounds. Seven wax compound classes were detected that had previously not been reported, and their structures were elucidated using gas chromatography-mass spectrometry of various derivatives. Six of the classes were identified as series of homologs di...

Fossil leaves which show excellent preservation of lipids and other organic compounds are preserved in abundance at the Miocene Clarkia lake deposit in northwestern Idaho, USA. Eighty three fossils from nine genera which are commonly found at the site, namely Quercus, Platanus, Magnolia, Pseudofagus, Fagus, Cocculus, Taxodium, Metasequoia and Sequoia were investigated to assess whether their ch...

Thermophysical properties of mixtures fatty acid esters with alkanols were measured in the whole composition range as a function temperature for understanding features biodiesel blends. Excess and mixing calculated from experimental measurements allowed to quantify analyze intermolecular forces considered systems. Likewise, molecular modelling studies using quantum chemistry classical dynamics ...

Gating of voltage-gated K(+) channels (K(v) channels) depends on the electromechanical coupling between the voltage sensor and activation gate. The main activation gate of K(v) channels involves the COOH-terminal section of the S6 segment (S6-b) and the S4-S5 linker at the intracellular mouth of the pore. In this study, we have expanded our earlier work to probe the concerted contribution of th...

In this study, the densities, excess molar volumes and partial molar volumes of four binary systems containing N,N-dimethyl acetamide (DMAc) with 1-alkanols (1-butanol up to 1-heptanol) are measured at 298.15 K. The results showed both constructive and expansive excess volumetric behavior for studying binary mixtures. Deviation values of heavy alcohol have more positive and less negative excess...