Fabry disease is a rare, inherited disease with lack of the enzyme alpha-galactosidase A (α-Gal)  in the cells of the body that participates in the breakdown of fat. The disease begins in early childhood, progresses slowly throughout life and results in severe damage of the kidneys, heart and central nervous system. The disease is life-threatening and if left untreated, death ...

The beta-galactosidase from the Antarctic gram-negative bacterium Pseudoalteromonas haloplanktis TAE 79 was purified to homogeneity. The nucleotide sequence and the NH(2)-terminal amino acid sequence of the purified enzyme indicate that the beta-galactosidase subunit is composed of 1,038 amino acids with a calculated M(r) of 118,068. This beta-galactosidase shares structural properties with Esc...

GMM1-ganglioside fi-galactosidase A (EC 3.2.1.23) has been purified 17,000-fold from human liver. The enzyme appeared as a single band of protein on polyacrylamide gel electrophoresis. Double immunodiffusion of rabbit anti-pgalactosidase A antiserum against either GM1 /3-galactosidase A or liver supematant gave a single precipitin band. The apparent molecular weight was 65,000 to 75,000 by gel ...

TROJANOVÁ I., RADA V. (2005): Enzymatic activity in fermented milk products containing bifidobacteria. Czech J. Food Sci., 23: 224–229. Streptococcus thermophilus, Lactobacillus delbrueckii subsp. bulgaricus and Bifidobacterium animalis strains were tested for α-galactosidase, β-galactosidase and α-glucosidase activities. Commercially available yoghurts with bifidobacteria were also tested. Whi...

crude enzyme (beta-galactosidase) extract (cee) was produced by lactobacillus ssp. bulgaricus chr hansen lb-12 and was applied in sterile milk which had been processed through ultra high temperature method (uht milk), for hydrolyzing lactose. lactosehydrolyzed milk was also produced by a pure and commercially available betagalactosidase (maxilact). optimum quantities of cee and maxilact enz...

OBJECTIVE To construct four recombinant Lactococcus lactis strains exhibiting high beta-galactosidase activity in fusion or non-fusion ways, and to study the influence factors for their protein expression and secretion. METHODS The gene fragments encoding beta-galactosidase from two strains of Lactobacillus bulgaricus, wch9901 isolated from yogurt and 1.1480 purchased from the Chinese Academy...

A plasmid containing promoter-deleted inactive /?-galactosidase gene [1] was used to select promoters of the pEP121 plasmid [2]. Colonies of cells harboring reactivated /?-galactosidase gene were identified by their red color on McConkey plates. The quantitative amounts of /?-galactosidase produced in each clone were estimated by assaying enzyme activity and by measuring the specific /?-galacto...

Rat brain cerebrosidase was shown by centrifugal fractionation to be a lysosomal enzyme. The pH optimum was found to be 4.5. Taurocholate greatly stimulated its action. The addition of palmitic acid had little effect on the enzyme activity, but ceramide, sphingosine, and ceramide lactoside inhibited. Galactose, galactonolactone, and galactitol inhibited the enzyme, but the corresponding compoun...

Ashbya gossypii has been recently considered as a host for the expression of recombinant proteins. The production levels achieved thus far were similar to those obtained with Saccharomyces cerevisiae for the same proteins. Here, the b-galactosidase from Aspergillus niger was successfully expressed and secreted by A. gossypii from 2-mm plasmids carrying the native signal sequence at higher level...