Low-level and unstable transgene expression are common issues using the CHO cell expression system. Matrix attachment regions (MARs) enhance transgene expression levels, but additional research is needed to improve their function and to determine their mechanism of action. MAR-6 from CHO chromosomes actively mediates high and consistent gene expression. In this study, we compared the effects of...

In this paper, we demonstrate a novel carbon nanotube (CNT) reinforced macroporous adsorption resin (MAR) for the first time. The CNTs were dispersed in water via sonication, and then in situ physically entrapped in the pores of MAR by capillary forces and sonication. The resulting CNT reinforced MAR (CNT-MAR) was proved by transmission electron microscopy (TEM) and confocal laser scanning micr...

In this study, gamma rays were used to irradiate embryogenic calli induced from cotyledon explants of Panax ginseng Meyer. After the embryogenic calli were irradiated, they were transferred to adventitious roots using an induction medium; next, mutated adventitious root (MAR) lines with a high frequency of adventitious root formations were selected. Two MAR lines (MAR 5-2 and MAR 5-9) from the ...

Background: Malnutrition in children including lower nutrient intakes or high density food intakes is of recent world concerns; therefore, determining a simple index that reveals the diet adequacy might be an advantage. Methods: This cross-sectional study was done among 233 children aged 2-5 years old. A 24-hour dietary questionnaire was used to calculate mean adequacy ratio (MAR), naturally nu...

Introduction: The precise mechanism for antisperm antibody (ASA) mediated fertility impairment is unclear. In the male reproductive tract, ASA may have an adverse impact on sperm maturation and function or overall semen quality. In this study, the percentage of ASA-IgA was determined by the direct Mixed Agglutination Reaction (MAR) test in men from infertile couples in Khorramabad city. Further...

The dosimetric impact of orthopedic metal artifact reduction (O-MAR) on spine SBRT patients has not been comprehensively studied, particularly with spinal prostheses in high-dose gradient regions. Using both phantom and patient datasets, we investigated dosimetric effects of O-MAR in combination of various metal locations and dose calculation algorithms. A physical phantom, with and without a t...

: one of the main classifications in human societies is based on sex. although this kind of classification is originated from the physiology of human being but it can result in some stereotypes about men and women. stereotypes are traits which can be attributed to all members of a group without considering the differences between them. in other words stereotypes are part of the thoughts and bel...

The aim of the present study was to investigate the effect of the characteristic elements of matrix attachment region (MAR) on transgene expression. Human β‑globin MAR was obtained by PCR amplification. A splicing MAR fragment containing all the characteristic elements of β‑globin MAR was artificially synthesized and then cloned into the eukaryotic expression vector. Following digestion and seq...

The importance of the mean annual runoff (MAR)-hydrological variable is paramount for catchment planning, development and management. MAR depicts the amount of uncertainty or chaos (implicitly information content) of the catchment. The uncertainty associated with MAR of quaternary catchments (QCs) in the Upper Vaal catchment of South Africa has been quantified through Shannon entropy. As a resu...