There is currently only one virus widely accepted for low-level containment or field release in the study of biological detection, the bacteriophage MS2. While MS2 has been used for decades in the bio-defense research, development, testing, and evaluation communities in lieu of actual viral threat agents, MS2 is less than ideal for several reasons. It is not the same size as most of the recogni...

Survey-scan-based label-free method have shown no compelling benefit over fragment ion (MS2)-based approaches when low-resolution mass spectrometry (MS) was used, the growing prevalence of high-resolution analyzers may have changed the game. This necessitates an updated, comparative investigation of these approaches for data acquired by high-resolution MS. Here, we compared survey scan-based (i...

BACKGROUND Real-time reverse transcription polymerase chain reaction (RT-PCR) is routinely used to detect viral infections. In Brazil, it is mandatory the use of nucleic acid tests to detect hepatitis C virus (HCV), hepatitis B virus and human immunodeficiency virus in blood banks because of the immunological window. The use of an internal control (IC) is necessary to differentiate the true neg...

The detection and quantification of enteric RNA viruses is based on isolation of viral RNA from the sample followed by quantitative reverse transcription polymerase chain reaction (RT-qPCR). To control the whole process of analysis and in order to guarantee the validity and reliability of results, process control viruses (PCV) are used. The present article describes the process of preparation a...

Evidence has been presented' for the accumulation of a double-stranded replicative form of MS2 RNA in vivo, following infection of Escherichia coli with MS2 phage, and for the identity of this material with the double-stranded RNA which is labeled in vitro with radioactive carbon2 on incubation of CL4-labeled ribonucleoside triphosphates with RNA synthetase.3 The present paper is concerned with...

Batch sorption experiments were carried out with the bacteriophages MS2 and phi X174. Two types of reactor vessels, polypropylene and glass, were used. Consistently lower concentrations of MS2 were found in the liquid phase in the absence of soil (control blanks) than in the presence of soil after mixing. High levels of MS2 inactivation (approximately 99.9%) were observed in control tubes made ...

INTRODUCTION Traditional methods of screening plant extracts and purified components for antiviral activity require up to a week to perform, prompting the need to develop more rapid quantitative methods to measure the ability of plant based preparations to block viral replication. We describe an adaption of an MS2 plaque reduction assay for use in S. aureus. RESULTS MS2 bacteriophage was capa...

Typically, detection of protein sequences in collision-induced dissociation (CID) tandem MS (MS2) dataset is performed by mapping identified peptide ions back to protein sequence by using the protein database search (PDS) engine. Finding a particular peptide sequence of interest in CID MS2 records very often requires manual evaluation of the spectrum, regardless of whether the peptide-associate...