epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant dna techniques. the aim of this study was to sub-clone the peroxisomal protein (pep) cdna into a mammalian expression vector leading to the formation of a  chimeric pep-cdna containing the flag epitope. the flag-pep recombinant cdna was construc...

Diversity-oriented synthesis (DOS) is an important field involving the synthesis of libraries of diverse small molecules for applications including biological screening. The last decade has witnessed major progress in the field, with continued emphasis on structural and functional diversity for DOS library construction. A number of recent reviews have updated the community on this topic, includ...

background: the aim of this study was to clone pparγ1 cdna in an appropriate mammalian expression vector, with a chimeric cdna form, encompassing pparγ with enhanced green fluorescent protein (egfp) cdna. this recombinant plasmid will be used for further analyses to investigate the molecular mechanism of pparγ1 for neural differentiation process. moreover, the nuclear localization of the pparγ1...

Suppression subtractive hybridization (SSH) is a widely used method for separating DNA molecules that distinguish two closely related DNA samples. Two of the main SSH applications are cDNA subtraction and genomic DNA subtraction. In fact, SSH is one of the most powerful and popular methods for generating subtracted cDNA or genomic DNA libraries. The SSH method is based on a suppression PCR effe...

tree nuts, including almond (prunus dulcis) are a source of food allergens often associated with life-threatening allergic reactions in susceptible individuals. although the proteins in almonds have been biochemically characterized, relatively little has been reported regarding the identity of the allergens involved in almond sensitivity. the present study was undertaken to identify the allerge...

A label-free colorimetric method based on exonuclease I (Exo I)-assisted signal amplification with protamine as a medium was developed for analysis of kanamycin. In this study, double-stranded DNA (dsDNA) probe tailored by manipulating an aptamer and its complementary (cDNA) ensuring detection target high selectivity excellent sensitivity. Herein, could not only combine negatively charged gold ...

NBS-LRR-type disease resistance gene-like cDNA, induced by salicylic acid (SA) was cloned from rye Secalecereale L. (2n = 14RR) var. Petkus, which has rust genes such as Lr26, Sr31 andRr9. We designed primers based on the NBS region and performed PCR using Petkus genomic DNA a template. Next, we TA-cloned 532-bp fragment containing five homologous amino sequences in region. The SA-treated showe...

عوامل متعددی بر روند تاثیرگذاری نگاره های ایران شبه قاره هند در دوران حکومت مغولان موثر بوده است. موضوع این پژوهش بررسی نگارگری دوره با منابع کتابخانه ای و بکارگیری روش توصيفی- تحليلی رویکرد تاریخی، آثار از منظر شیوه پیدایش، ساختار، خصایص صوری تاثیرپذیری هنر ایرانی مورد قرار گرفته ‏است. اساس نتایج پژوهش، عصر مغولان، به گونه چشم گیر معرف نفوذ طور کلی، گرایش هنرمندان مغول هنرهای تصویری ایران‏ قوا...