different grapevine cultures that were inoculated with Plasmopara viticola as a host pathogen and Pseudoperonospora cubensis as a non-host pathogen. The results of the expression analysis showed that the PR1 mRNA level in Vitis vinifera cv. Riesling and Vitis riparia cv. Gloire de Montpellier is mainly affected by the culture system. PR1 is constitutively expressed in callus cultures. In vitro ...

We describe the safety and immunogenicity of a combined vaccine of 2 leukemia-associated antigenic peptides, PR1 and WT1. Eight patients with myeloid malignancies received one subcutaneous dose each of PR1 and WT1 vaccines in Montanide adjuvant, with granulocyte-macrophage colony-stimulating factor. Patients were reviewed weekly for 4 weeks to monitor toxicity and immunologic responses. Toxicit...

PR1 (VLQELNVTV) is an HLA-A2-restricted leukemia-associated peptide from proteinase 3 and neutrophil elastase that is recognized by PR1-specific cytotoxic T lymphocytes that contribute to cytogenetic remission of myeloid leukemia. We report a novel T cell receptor (TCR)-like IgG2a antibody (8F4) with high specific binding affinity (KD=9.9nM) for a combined epitope of the PR1/HLA-A2 complex. Flo...

Previous investigations have demonstrated that a synthetic retinoid, N-(4-hydroxyphenyl) retinamide (4-HPR), inhibits the invasion of prostate adenocarcinoma in vitro. Urokinase-type plasminogen activator (uPA) is a prerequisite for tumor invasion. The purpose of this study was to evaluate the effects of 4-HPR on uPA and plasminogen activator inhibitor-1 (PAI-1) in prostate cancer. Human prosta...

Repeated subculturing caused rapid changes in the spore surface properties and virulence of Metarhizium anisopliae. Of the two strains evaluated, M. anisopliae V245 attenuated more rapidly than V275. Electrophoretic mobility and Radial Flow Chamber assays were used for the first time to generate qualitative and quantitative information on the adhesive forces of M. anisopliae conidia. Independen...

2 ∥∥∥(x∗ − x̃) + σ n z̃− δ∥∥2l2 s.t. δ ∈ C − x̃. Letting R1 and R2 denote orthogonal subspaces that contain Q1 and Q2, i.e., Q1 ⊆ R1 and Q2 ⊆ R2, and letting δ = PR1(δ), δ (2) = PR2(δ), δ̂ (1) n (C) = PR1(δ̂n(C)), δ̂ (2) n (C) = PR2(δ̂n(C)) denote the projections of δ, δ̂n(C) onto R1, R2, we can rewrite the above reformulated optimization problem as: [ δ̂ (1) n (C), δ̂ (2) n (C) ] = arg min δ∈Q1,δ∈Q2 1 2...

Both intranasal (i.n.) and intracerebral (i.c.) inoculation of mice with wild-type equine herpesvirus type 1 (wt EHV-1) caused clinical signs and mortality. Virus could be recovered from target organs (turbinates, lungs and blood) for several days. By contrast, the thymidine kinase (TK)-deficient deletion mutant PR1 produced markedly less clinical disease following both i.n. and i.c. inoculatio...

BACKGROUND We previously showed that vaccination with one dose of PR1 and WT1 peptides induces transient anti-leukemia immunity. We hypothesized that maintenance of a sustained anti-leukemia response may require frequent boost injections. DESIGN AND METHODS Eight patients with myeloid malignancies were enrolled in this phase II study, and 6 completed 6 injections of PR1 and WT1 peptides in Mo...