glucose 6- phosphate dehydrogenase from streptomyces aureofaciens was purified andinactivated by pyridoxal 5′-phosphate (plp). the inactivation was a pseudo-first order and time-dependentreaction. complete inactivation was achieved at 0.2mm plp within 16 minutes. the type of inhibition wascompetitive with respect to glucose 6- phosphate. spectral characteristics of plp-enzyme complexcorresponde...

Ilizarov is an accepted technique for lengthening short amputation stumps to improve prosthetic function andrehabilitation. The relation of stump lengthening and phantom limb pain (PLP) has not been reported in literature.We present here a case report of a transfemoral amputee who had a flare up of PLP following stump lengthening.He responded well to a combination of pharmacological therapy and...

Effective learning is an important aspect for students as a provision the introduction of field schooling (PLP) students. The purpose this study was to determine how much can apply effective models in process when following PLP, well application these affect effectiveness PLP. design research conducted qualitative with descriptive approach. population many 55 and then sampled number 30 random s...

The parathyroid hormone-like peptide (PLP) gene is widely expressed in normal and neoplastic tissues. Previous studies have demonstrated that PLP gene expression is regulated by serum and cycloheximide, features common to the regulation of a number of different early response genes. We now report that PLP mRNA transcripts are induced within 5 min of exposure of rat keratinocytes to serum, retur...

PRL-like protein C variant (PLP-Cv) is a newly identified member of the PRL family. PLP-Cv is specifically expressed in the chorioallantoic placenta by two distinct cell populations: trophoblast giant cells and spongiotrophoblast cells. To gain some insight regarding the control of PLP-Cv gene expression and the regulatory factors controlling trophoblast giant cell and spongiotrophoblast cell l...

We have examined circulating concentrations of a parathyroid hormone-like peptide (PLP) in patients with malignancies and in patients with hyperparathyroidism. The radioimmunoassay employed reacts with synthetic amino-terminal fragments of PLP but not with parathyroid hormone. Elevated plasma PLP concentrations were observed in 50% of patients with malignancy and hypercalcemia and in 15% of nor...

Pyridoxal 5'-phosphate (PLP) is a cofactor for dozens of B(6) requiring enzymes. PLP reacts with apo-B(6) enzymes by forming an aldimine linkage with the ε-amino group of an active site lysine residue, thus yielding the catalytically active holo-B(6) enzyme. During protein turnover, the PLP is salvaged by first converting it to pyridoxal by a phosphatase and then back to PLP by pyridoxal kinase...

Recombinant methioninase (rMETase) has been shown to target the elevated methionine (MET) dependence of tumor cells and arrest their growth as well as make tumors more sensitive to standard chemotherapy agents. Polyethylene glycol (PEG)-modified rMETase (PEG-rMETase) has reduced antigenicity compared with unmodified rMETase. However, PEG-rMETase has a limited active circulating half-life due to...

The prolactin (PRL) family consists of a collection of genes expressed in the uterus, placenta and anterior pituitary. These cytokines/hormones participate in the control of maternal-fetal adaptations to pregnancy. In this report, we establish the presence of three new members of the PRL family. Novel expressed sequence tags (ESTs) with homology to PRL were isolated from embryonic and placental...

PURPOSE To examine whether there is central sensitization in patients with phantom limb pain (PLP) after traumatic limb amputation. METHODS Seventeen patients after unilateral lower limb amputation secondary to trauma were enrolled. Ten patients had chronic PLP, while the other seven patients had no PLP. Tactile-sensation threshold, cold- and warm-sensation thresholds, cold- and heat-pain thr...