Applying directed evolution to the phloroglucinol synthase PhlD from Pseudomonas fluorescens Pf-5 has provided the first example of engineering enhanced productivity in a type III polyketide synthase, and a rare instance of improving the activity of a biosynthetic enzyme from secondary metabolism.

ABSTRACT Pseudomonas fluorescens strains producing the antibiotic 2,4-diacetylphloroglucinol (DAPG) have biocontrol activity against a broad spectrum of root and seedling diseases. In this study, we determined the effect of genotype on the ability to isolate and quantify introduced 2,4-DAPG producers from the rhizosphere of wheat using three different methods: traditional dilution plating on se...

There is a significant potential to improve the plant-beneficial effects of root-colonizing pseudomonads by breeding wheat genotypes with a greater capacity to sustain interactions with these bacteria. However, the interaction between pseudomonads and crop plants at the cultivar level, as well as the conditions which favor the accumulation of beneficial microorganisms in the wheat rhizosphere, ...

The aim of this study was to inventory the types of plant growth-promoting rhizobacteria (PGPR) present in the rhizosphere of plants grown in soils contaminated with heavy metals, recalcitrant organics, petroleum sewage or salinity in China. We screened 1223 isolates for antifungal activity and about 24% inhibited Rhizoctonia solani or Sclerotinia sclerotiorum. Twenty-four strains inhibitory to...

We assessed a collection of 47 fluorescent Pseudomonas spp., some with known biological     control activity against certain soil-borne phytopathogenic fungi such as, Macrophomina phaseolina, Rhizoctonia solani, Phytophthora nicotianae var. parasitica, Pythium sp. and  Fusarium sp. in vitro and the potential to produce known secondary metabolites such as, siderophore, HCN and protease. The resu...

 It is one of the most nutritious millet and staple food in different states of India and Pseudomonas sp. MSSRFD41  MSSRFD41 was isolated from finger millet rhizosphere soil samples. 16S rRNA gene sequence shows maximum 98.77% similarity and forms monophyletic clade with closely related species.  It produces 2,4-DAPG, HCN, AHLs, IAA, solubilises phosphate and tolerates 1 M NaCl.  Exhibits p...

Pseudomonas fluorescens 2P24 is a soilborne bacterium that synthesizes and excretes multiple antimicrobial metabolites. The polyketide compound 2,4-diacetylphloroglucinol (2,4-DAPG), synthesized by the phlACBD locus, is its major biocontrol determinant. This study investigated two mutants defective in antagonistic activity against Rhizoctonia solani. Deletion of the gidA (PM701) or trmE (PM702)...

we assessed a collection of 47 fluorescent pseudomonas spp., some with known biological     control activity against certain soil-borne phytopathogenic fungi such as, macrophomina phaseolina, rhizoctonia solani, phytophthora nicotianae var. parasitica, pythium sp. and  fusarium sp. in vitro and the potential to produce known secondary metabolites such as, siderophore, hcn and protease. the resu...