background staphylococcus aureus is a significant pathogen that can colonize the nares of different animals, causing a wide range of infections in various hosts. objectives we intended to determine the prevalence of s. aureus in the nasal cavity of healthy ruminants and also to investigate the presence of antibiotic resistance genes. materials and methods in the present study, healthy cattle (n...

The aim of this study was to investigate the presence of virulence genes in 46 coagulase-negative staphylococci (CNS) strains isolated from 46 udder quarters of 46 cows with subclinical mastitis. The isolates were examined phenotypically (cultural features, Gram stain, catalase, coagulase, API tests) and genotypically for: icaA, icaAB, icaD, atlE, bhp, agrA, sarA, mecA, and blaZ genes. These is...

Using blaZ PCR as the "gold standard," the sensitivities of CLSI penicillin zone edge and nitrocefin-based tests for β-lactamase production in Staphylococcus aureus were 64.5% and 35.5%, respectively, with specificity of 99.8% for both methods. In 2013, 13.5% of 3,083 S. aureus isolates from 31 U.S. centers were penicillin susceptible.

OBJECTIVES The mec and bla systems, among other genetic factors, are critical in regulating the expression of methicillin resistance in Staphylococcus aureus. We examined by WGS a naturally occurring oxacillin-susceptible mecA-positive S. aureus isolate to identify the mechanism conferring oxacillin susceptibility. METHODS The mecA-positive oxacillin-susceptible S. aureus isolate GR2 (penicil...

ABSTRACT Bovine clinical mastitis caused by Staphylococcus spp. is a serious and widespread disease in the world of dairy farming. Antimicrobial therapy fundamental importance prevention treatment infectious mastitis, but indiscriminate use antimicrobials acts as determining factor for spread disease. The present study evaluated resistance profiles 57 isolated from bovine to beta-lactams gentam...

Patients with Active and Quiescent Crohn Disease and Ulcerative Colitis: A Chemiluminescent Study, Anna Blázovics, Ágota Kovács, Andrea Lugasi, Krisztina Hagymási, Lajos Bı́ró, and János Fehér ( Second Department of Medicine, Semmelweis University Medical School, Szentkirályi St. 46, Budapest H-1088, Hungary; 2 Elizabeth County Hospital, Alsó erdõsor St. 7, Budapest H-1074, Hungary; 3 National I...

The aim of this study was to determine the presence methicillin resistant staphylococci (MRS) in retail raw chicken meat samples sold Hatay. antimicrobial susceptibility isolates tested for 13 different antimicrobials by disc diffusion method and investigated resistance genes encoding (mecA), tetracycline (tetM, tetK), penicillin (blaZ), macrolide (ermA, ermC), lincosamide (lnuA) aminoglycoside...

We describe here the isolation of 8 beta-lactamase-producing multidrug-resistant Enterococcus faecium isolates in 2010. All strains showed diverse pulsed-field gel electrophoresis (PFGE) profiles, all belonging to the same clonal complex, CC17. By PCR and hybridization experiments, the entire blaZ-blaI-blaR1 operon was found. The beta-lactamase activity was demonstrated at a high inoculum and i...

The sequence of methicillin-resistant Staphylococcus aureus strain B6 (sequence type 30 [ST30], spa type t433, staphylococcal chromosomal cassette mec element [SCCmec] type IVc, Panton-Valentine leukocidin [PVL] positive), isolated from a pediatric patient with a lung infection in Niterói, Rio de Janeiro, Brazil, is described here. The draft genome sequence includes a 2.8-Mb chromosome, accompa...