Infections due to multidrugand carbapenem-resistant isolates of Acinetobacter baumannii were frequent among patients treated in the Tzannion General Hospital in Piraeus, Greece, throughout the period 2005 to 2006. Six of these isolates, derived from blood cultures of patients in three different wards (four were from patients in the intensive care unit) and taken at least 1 month apart from each...

The increase in the prevalence of carbapenem-resistant Pseudomonas aeruginosa isolates, particularly caused by the metallo-ß-lactamases (MBL) is of great concern in the clinical settings worldwide. Here, we report the detection and identification of MBL producing P. aeruginosa in our hospital (University Malaya Medical Center, Malaysia), and the characterization of MBL cassette-containing integ...

23 We describe the occurrence of blaVIM-2 in 10 carbapenem-resistant Pseudomonas monteilii 24 strains isolated from different clinical samples in our hospital in Northern Spain. All the 25 blaVIM-2-harbouring P. monteilii isolates possessed a class 1 integron, with the cassette array 26 [intI1_blaVIM-2_aac(6’)-Ib_qacE1_sul1]. Our results show the emergence of VIM-227 producing multidrug-resist...

Results: The blaVIM gene was present in 34 (43.6%), blaIMP in five (6.4%) and none of the isolates from 2008 had blaNDM-1 or blaKPC genes. Among the isolates from 2012, blaNDM-1 gene was present in 47 (53.4%), blaVIM in 19 (24.4%), blaIMP in one (1.1%) and none had blaKPC. There were nine isolates during the two years which had multiple genes encoding carbapenemases; while 66 did not have any o...

OBJECTIVES Acinetobacter baumannii is a significant pathogen in health care settings. In recent years, an increase in carbapenem resistance among A. baumannii due to Ambler class B metallo-beta-lactamases or class D OXA carbapenamases has been reported. In this study we detected the presence of OXA carbapenamases and coproduction of metallo-beta-lactamases (blaVIM and blaIMP ) by phenotypic and...

Background: Infectious by Pseudomonas aeruginosa has spread worldwide and metallo-beta-lactamases (MBL) are being reported with increasing frequency. The aim of this study was to investigate the antibiotic susceptibility and distribution of blaVIM and blaIMP genes in P. aeruginosa isolates from Zanjan Province of Iran. Methods: A total of 70 P. aeruginosa isolates were identified from patients ...

23 We describe the occurrence of blaVIM-2 in 10 carbapenem-resistant Pseudomonas monteilii 24 strains isolated from different clinical samples in our hospital in Northern Spain. All the 25 blaVIM-2-harbouring P. monteilii isolates possessed a class 1 integron, with the cassette array 26 [intI1_blaVIM-2_aac(6’)-Ib_qacE1_sul1]. Our results show the emergence of VIM-227 producing multidrug-resist...

©2011 Elsevier Editora Ltda. All rights reserved. PCR analyses were performed with whole genomic DNA from all case isolates. Primers used for analysis of blaVIM /blaIMP genes were Vim F/R, Imp F/R respectively. PCR primers were designed and PCR conditions were performed as previously described.3 Association of integrons with MBL genes was confirmed by PCR using combination of blaand integron-sp...

Carbapenem-resistant Enterobacteriaceae encountered in countries of the Arabian Peninsula usually produce OXA-48-like and New Delhi metallo-beta-lactamases (NDM) carbapenemases. However, a temporary increase in VIM-4-producing, clonally unrelated Enterobacteriaceae strains was described earlier in a Kuwaiti hospital. We investigated the genetic support of blaVIM-4 in six Klebsiella pneumoniae s...

The objective of this study was to evaluate the susceptibility of metallo-β-lactamase (MBL)-producing Acinetobacter baumannii (A. baumannii) clinical isolates to biocides. We also determined the prevalence and correlation of efflux pump genes, class 1 integron and MBL encoding genes. In addition, blaVIM, blaNDM-1, qacE and qacEΔ1 nucleotide sequence analysis was performed and compared to sequen...