more than 185 species, strains and unclassified theileria parasites are categorized in the entrez taxonomy. the accurate diagnosis and proper identification of the causative agents are important for understanding the epidemiology, prevention and appropriate treatment. this study aims to discuss the importance of two genes of theileria annulata 18s ribosomal rna (18s rrna) and theileria annulata...

The loop of a stem structure close to the 5' end of the 18S rRNA is complementary to the box A region of the U3 small nucleolar RNA (snoRNA). Substitution of the 18S loop nucleotides inhibited pre-rRNA cleavage at site A(1), the 5' end of the 18S rRNA, and at site A(2), located 1.9 kb away in internal transcribed spacer 1. This inhibition was largely suppressed by a compensatory mutation in U3,...

background: various fixation and permeabilization techniques have been developed for detection of intracellular antigens by flow cytometry; however, there are few studies using flow cytometry to detect the frequency of intracellular nucleic acids, particularly rna. we tested six different permeabilization methods in order to gain access to a high quality method with minimal damage to intracellu...

Several families in the beetle suborder Adephaga have an aquatic life style and are commonly grouped in the “Hydradephaga,” but their monophyly is contentious and relationships between and within these families are poorly understood. Here we present fulllength 18S rRNA sequence for 84 species of Hydradephaga, including representatives of most major groups down to the tribal level, and a total o...

The naturally occurring modified nucleoside 3-[3-amino-3-carboxypropyl]-1-methylpseudouridine (abbreviated am psi) is found in eukaryotic 18S rRNA. We localized am psi to sequence resolution in D. melanogaster 18S rRNA. This hypermodified base causes an absolute stop in cDNA elongation. The RNA sequence bearing am psi was determined by dideoxy-sequencing with reverse transcriptase. The rDNA cod...

The H/ACA RNAs represent an abundant, evolutionarily conserved and functionally diverse class of non-coding RNAs. Many H/ACA RNAs direct pseudouridylation of rRNAs and snRNAs, while members of the rapidly growing group of 'orphan' H/ACA RNAs participate in pre-rRNA processing, telomere synthesis and probably, in other nuclear processes. The yeast snR30 'orphan' H/ACA snoRNA has long been known ...

Intermolecular hybridization experiments show that murine 18S rRNA and 28S rRNA are capable of forming stable hybrid structures with mRNA from genes p53, c-myc and c-mos from the same species. Both 5'-uncoding and coding oncogene p53 mRNA regions contain fragments interacting with rRNA. Computer analysis revealed 18S rRNA fragments complementary to oligonucleotides frequently met in mRNA, which...

Abstract Faeces from cows with different milk yield and non-lactating were applied to soil investigate whether N 2 O efflux is related feeding-induced differences in faecal microbiome abundances of nitrification denitrification genes. Fungal 18S-rRNA gene abundance was the highest faeces group. The 18S-rRNA/ergosterol ratio showed a strong positive correlation 18S-rRNA/fungal glucosamine ratio....

Biodiversity studies are commonly conducted using 18S rRNA genes. In this study, we compared the inter-species divergence of variable regions (V1-9) within the copepod 18S rRNA gene, and tested their taxonomic resolutions at different taxonomic levels. Our results indicate that the 18S rRNA gene is a good molecular marker for the study of copepod biodiversity, and our conclusions are as follows...