Introduction: In patients undergoing surgery for mitral valve replacement (MVR) for valvular heart disease, pulmonary artery hypertension (PAH) has been considered a major risk factor. In this prospective study, we have studied the early hemodynamic changes and post-operative outcomes of MVR among patients with severe PAH. Methods: 68 consecutive patients who underwent mitral valve replacement ...

BACKGROUND Prostatic acid phosphatase (PAP) and ecto-5'-nucleotidase (NT5E, CD73) produce extracellular adenosine from the nucleotide AMP in spinal nociceptive (pain-sensing) circuits; however, it is currently unknown if these are the main ectonucleotidases that generate adenosine or how rapidly they generate adenosine. RESULTS We found that AMP hydrolysis, when measured histochemically, was ...

phosphatidate phosphohydrolase (pap) from cytosolic fraction of rat liver was purified to homogeneity having specific activity of 5.14 u/mg protein. an activity staining procedure was developed to determine molecular weight of the enzyme on polyacrylamide gel electrophoresis using ferguson plot. molecular weight (m.w.) of the active pap was 298 kda. sds-page analysis showed a m.w. of 47 kda for...

The expression of Pap pili that facilitate the attachment of Escherichia coli to uroepithelial cells is shut off outside the host at temperatures below 26 degrees C. Ribonuclease protection analysis showed that this thermoregulatory response was rapid as evidenced by the absence of papBA transcripts, coding for Pap pilin, after only one generation of growth at 23 degrees C. The histone-like nuc...

Using PC12 cells as an in vitro model system, we have identified a series of transcripts induced through activation of the leptin receptor. On the basis of kinetic studies, two distinct gene sets could be discerned: signal transducer and activator of transciption-3 (STAT-3), suppressor of cytokine signalling-3 (SOCS-3), MT-II (metallothionein-II), the serine/threonine kinase fibroblast-growth-f...

Background: Bleeding during or immediately after the intercourse can be seen in women with benign cervical changes such as polyps, ectropion or infection of the cervix. But, sometimes, important diseases such as cancer of the cervix can be associated with post coital bleeding (PCB). The aim of this study was to find the prevalence of microscopic pre-cancerous lesions of the cer...

An experiment was performed to investigate the effect of animal vs. vegetable protein sources in the diet of laying hens on the development of hen performance. A diet containing protein sources of only vegetable origin was compared with 4 diets, each containing 1 of 4 processed animal proteins (PAP). Two PAP (Daka-58 and Sonac-60) were classified as meat meals, and the remaining 2 (Daka-40 and ...

Thrombotic thrombocytopenic purpura (TTP) is characterized by widespread platelet thrombi in arterioles and capillaries. Unusually large or multimeric von Willebrand factor, as well as one or more platelet-agglutinating factors, have been implicated in the pathogenesis of TTP. But, the actual mechanisms of platelet agglutination have not been satisfactorily explained. Recent studies suggested t...

The measurement was evaluated of serum prostate specific antigen (PSA) and prostatic acid phosphatase (PAP) levels for early detection of prostate cancer on a potentially high-risk group. A PSA level at 5.0 ng per ml and PAP at 4.0 ng per ml were chosen as the cut-off levels. Results obtained for PSA and PAP can be divided into three groups: Group I, with normal levels of PSA and PAP; Group II,...