نتایج جستجو برای: پروتئین hsp40

تعداد نتایج: 18516  

Journal: :Genetics 2011
Carolyn D Silflow Xiaoqing Sun Nancy A Haas Joseph W Foley Paul A Lefebvre

Mutations at the APM1 and APM2 loci in the green alga Chlamydomonas reinhardtii confer resistance to phosphorothioamidate and dinitroaniline herbicides. Genetic interactions between apm1 and apm2 mutations suggest an interaction between the gene products. We identified the APM1 and APM2 genes using a map-based cloning strategy. Genomic DNA fragments containing only the DNJ1 gene encoding a type...

2013
Daniel W. Summers Katie J. Wolfe Hong Yu Ren Douglas M. Cyr

Mechanisms for cooperation between the cytosolic Hsp70 system and the ubiquitin proteasome system during protein triage are not clear. Herein, we identify new mechanisms for selection of misfolded cytosolic proteins for degradation via defining functional interactions between specific cytosolic Hsp70/Hsp40 pairs and quality control ubiquitin ligases. These studies revolved around the use of S. ...

Journal: :The EMBO journal 2012
Heike Rampelt Janine Kirstein-Miles Nadinath B Nillegoda Kang Chi Sebastian R Scholz Richard I Morimoto Bernd Bukau

Accumulation of aggregation-prone misfolded proteins disrupts normal cellular function and promotes ageing and disease. Bacteria, fungi and plants counteract this by solubilizing and refolding aggregated proteins via a powerful cytosolic ATP-dependent bichaperone system, comprising the AAA+ disaggregase Hsp100 and the Hsp70-Hsp40 system. Metazoa, however, lack Hsp100 disaggregases. We show that...

Journal: :Chemistry & biology 2015
Masatsugu Hiraki So-Young Hwang Shugeng Cao Timothy R Ramadhar Sanguine Byun Kyoung Wan Yoon Jung Hyun Lee Kiki Chu Aditi U Gurkar Vihren Kolev Jianming Zhang Takushi Namba Maureen E Murphy David J Newman Anna Mandinova Jon Clardy Sam W Lee

TP53 is the most frequently mutated gene in human cancer, and small-molecule reactivation of mutant p53 function represents an important anticancer strategy. A cell-based, high-throughput small-molecule screen identified chetomin (CTM) as a mutant p53 R175H reactivator. CTM enabled p53 to transactivate target genes, restored MDM2 negative regulation, and selectively inhibited the growth of canc...

2015
Zhong-Yuan Li Jing Lu Dong-Hui Zhou Jia Chen Xing-Quan Zhu

Toxoplasma gondii with worldwide distribution has received substantial medical and scientific attentions as it causes serious clinical and veterinary problems especially for pregnant women and immunocompromised patients. Heat shock protein 40 (HSP40) plays a variety of essential roles in the pathogenesis of this protozoan parasite. In order to detail the genetic diversity of HSP40 gene, 16 T. g...

Journal: :Molecular biology of the cell 2005
Yuhkoh Satouh Potturi Padma Toshifusa Toda Nori Satoh Hiroyuki Ide Kazuo Inaba

Members of the heat-shock protein (HSP)40 regulate the protein folding activity of HSP70 proteins and help the functional specialization of this molecular chaperone system in various types of cellular events. We have recently identified Hsp40 as a component of flagellar axoneme in the ascidian Ciona intestinalis, suggesting a correlation between Hsp40 related chaperone system and flagellar func...

Journal: :The EMBO journal 2008
James Shorter Susan Lindquist

Self-templating amyloid forms of Sup35 constitute the yeast prion [PSI(+)]. How the protein-remodelling factor, Hsp104, collaborates with other chaperones to regulate [PSI(+)] inheritance remains poorly delineated. Here, we report how the Ssa and Ssb components of the Hsp70 chaperone system directly affect Sup35 prionogenesis and cooperate with Hsp104. We identify the ribosome-associated Ssb1:Z...

Journal: :Molecular biology of the cell 2005
Chun Yang Mark M Compton Pinfen Yang

The radial spoke is a stable structural complex in the 9 + 2 axoneme for the control of flagellar motility. However, the spokes in Chlamydomonas mutant pf24 are heterogeneous and unstable, whereas several spoke proteins are reduced differentially. To elucidate the defective mechanism, we clone RSP16, a prominent spoke protein diminished in pf24 axonemes. Unexpectedly, RSP16 is a novel HSP40 mem...

Journal: :Protein and peptide letters 2011
Addmore Shonhai Alexander G Maier Jude M Przyborski Gregory L Blatch

Certain kinetoplastid (Leishmania spp. and Tryapnosoma cruzi) and apicomplexan parasites (Plasmodium falciparum and Toxoplasma gondii) are capable of invading human cells as part of their pathology. These parasites appear to have evolved a relatively expanded or diverse complement of genes encoding molecular chaperones. The gene families encoding heat shock protein 90 (Hsp90) and heat shock pro...

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