Super-resolution optical fluctuation imaging (SOFI) is a super-resolution microscopy technique that overcomes the diffraction limit by analyzing intensity fluctuations of statistically independent emitters in time series images. The final images are background-free and show confocality enhanced spatial resolution (super-resolution). Fluorophore photobleaching, however, key limitation for record...

A straightforward method to achieve super-resolution consists of taking an image sequence of stochastically blinking emitters using a standard wide-field fluorescence microscope. Densely packed single molecules can be distinguished sequentially in time using high-precision localization algorithms (e.g., PALM and STORM) or by analyzing the statistics of the temporal fluctuations (SOFI). In a fac...

This paper present a novel algorithm for monotone front propagation of anisotropic nature. In several examples the new algorithm is shown to be fast and able to solve a general class of front propagation problems. The algorithm is inspired by Huygens’ principle in that the front is described using a list of nodes that are used as source points to evolve the front. Nodes affected by the source p...

Background: Approximately one-half of new nurses with less than one year of experience who were involved in adverse patient events identified that their formal education preparation was a causal factor in their error (Saintsing, Gibson & Pennington, 2011). An examination of quality and safety measures of a current hospital based associate degree nursing (ADN) educational program provided data r...

Background: Aging of the myocardium is a dynamic process which involves progressive loss of cardiomyocytes due to necrosis and apoptosis, interstitial fi brosis and reactive hypertrophy of the remaining vital cardiomyocytes. In our study, we investigated the postnatal changes in the myocardium of 15 adult male Wistar rats, distributed in the following age groups: 2 weeks, 1, 3, 6 and 12 months ...

By switching fluorophores on and off in either a deterministic [1, 2] or a stochastic [3–7] manner, superresolution microscopy has enabled the imaging of biological structures at resolutions well beyond the diffraction limit. Superresolution optical fluctuation imaging (SOFI) [7, 8] provides an elegant way of overcoming the diffraction limit in all three spatial dimensions by computing higher-o...

Abstract Super-resolution microscopy techniques have pushed-down the limits of resolution in optical imaging by more than an order magnitude. However, these methods often require long acquisition times addition to complex setups and sample preparation protocols. fluctuation (SOFI) emerged over ten years ago as approach that exploits temporal spatial correlations within acquired images obtain in...

The semi-ordered fast iterative method is used to compute a monotone front propagation of anisotropic nature by solving the eikonal equation. Compared to established iterative methods, such as the fast iterative method, the semi-ordered fast iterative method (SOFI) offers increased stability for variations in the front velocity. So far, the method has only been investigated in a serial, two-dim...