We used 30 genetic markers of 6 different classes to describe hierarchical genetic structure in introduced populations of the fire ant Solenopsis invicta. These included four classes of presumably neutral nuclear loci (allozymes, codominant random amplified polymorphic DNAs (RAPDs), microsatellites, and dominant RAPDs), a class comprising two linked protein-coding nuclear loci under selection, ...

PURPOSE To evaluate the validity of different methods of relative afferent pupillary defect (RAPD) detection and assess the usefulness of detecting an RAPD in glaucoma patients. METHODS For this prospective observational study, we enrolled 70 of 153 consecutive glaucoma patients coming for examination. Exclusion criteria were cloudy corneas, recent surgery, fixed pupils, and other diseases pr...

Evaluation of relationships is of prime importance in any breeding studies. In current research, genetic diversity among different rapeseed genotypes from several geographical origins including Canada, Australia, Germany, France, Denmark, Netherlands, Sweden, Iran, and Somegenotypesof unknown origin, were evaluated by RAPD and ISSR markers. All of the generated bands using 12 RAPDs and 15 ISSRs...

Sesame (Sesamum indicum L.) is an ancient oilseed crop known for its nutty seeds and high-quality edible oil. It is an unexplored crop with a great economic potential. The present study deals with assessment of genetic diversity in the crop. Twenty two RAPD and 18 SSR primers were used for analysis of the 47 different sesame accessions grown in different agroclimatic zones of India. A total of ...

The rapid development of molecular techniques offers a palette of technical approaches for population biologists interested in a wide range of questions. For example, these tools can be used to determine individual reproductive success or to measure rates of genetic divergence among populations. Which technique is most appropriate for a particular question depends upon (1) the extent of genetic...

Genetic markers facilitate the study of inheritance and the cloning of genes by genetic approaches. Molecular markers detect differences in DNA sequence, and are thus less ambiguous than phenotypic markers, which require gene expression. We have demonstrated a molecular approach to the mapping of mutant genes using RAPD markers and pooling of individuals based on phenotype. To map genes by phen...

ABSTRACT A three-generation American chestnut x Chinese chestnut pedigree was used to construct a genetic linkage map for chestnut and to investigate the control of resistance to Endothia parasitica (chestnut blight fungus). DNA genotypes for 241 polymorphic markers (eight isozymes, 17 restriction fragment length polymorphisms [RFLPs], and 216 random amplified polymorphic DNAs [RAPDs]) were ass...

in the present study, random amplified polymorphic dna (rapd) and inter-simple sequence repeat (issr) markers were used to estimate genetic diversity and relationships among 35 cluster bean [cyamopsis tetragonoloba (l.) taub] genotypes. for rapd analysis, 20 random primers were used which amplified 164 bands, 147 of which were polymorphic with an average polymorphism of 90.3%. the amplified pro...