Isoelectric focusing (IEF), agarose electrophoresis, and crossed immunoelectrophoresis (CIE) were used to resolve the heme-containing proteins of the Escherichia coli cytoplasmic membrane after solubilization by Triton X-100. Two bands in IEF stained for heme with pI values of 4.7 and 5.3. One of the bands, with an isoelectric point of pH 5.3, was present only when the cells were grown to late ...

Received 18 November 1997; accepted 27 June 1998) The procedure for phenotyping of most genetic variants in cow milk was optimised for iso-electric focusing (IEF) of camel milk proteins and milk from individual camels of different breeds was screened. The caseins obtained from IEF bands were also investigated by Nterminal sequencing. Camel milk casein was separated at different pH and the prote...

This paper reports a protocol that improves the resolving power of isoelectric focusing (IEF) in a polymeric microfluidic chip. This method couples several stages of IEF in series by first focusing proteins in a straight channel using broad-range ampholytes and then refocusing segments of the first channel into secondary channels that branch from the first one at T-junctions. Experiments demons...

The occurrence of charge variants of phosphohexose isÃ3meras? was elucidated in cancerous and, for comparison, in noncancerous tissues obtained from the same organ. Surgical specimens from 35 patients with gastrointestinal and mammary carcinoma were studied by means of the isoelectric focusing (IEF) technique. Differences in the IEF pattern could be demonstrated in 90% of the patients with gast...

Distinct genetic variants of apolipoprotein (apo) Al have been shown to influence concentrations of high-density lipoprotein (HDL) cholesterol. The genetic polymorphism of apo AIV may modulate HDL-cholesterol, plasma triglycerides, and lipoprotein(a) concentrations. There is evidence for an antagonizing role of apo All in reverse cholesterol transport. Since genetic polymorphisms and variants o...

The birth and evolution of IEF in conventional carrier ampholyte buffers is reviewed here, from a shaky start during World War II, via desperate attempts of Svensson to create pH gradients by stationary electrolysis of salts, to the development of the IEF theory and the solution of the steady-state equation. The remarkable synthetic process of Ampholines, as ingeniously devised by Vesterberg, i...

This paper describes fabrication of glass and plastic microfluidic devices for protein separations. Although the long-term goal is to develop a microfluidic device for two-dimensional gel electrophoresis, this paper focuses on the first dimension–isoelectric focusing (IEF). A laserinduced fluorescence (LIF) imaging system has been built for imaging an entire channel in an IEF device. The whole-...

This paper reports a simple isoelectric focusing (IEF) technique without carrier ampholytes to generate stable pH gradients in a nitrocellulose membrane for the fabrication of paper-based analytical devices suitable for complex biological samples. To design the custom-built IEF apparatus, silver-coated copper wire electrodes were employed; the distance between anode and cathode was 8 mm. Concen...

We prepared Bi3O4Cl single-crystalline nanosheets with high {001} facet exposure percentages and demonstrated that their photoreactivity strongly depended on the magnitude of the internal electric field (IEF), which was correlated with the {001} facet exposure percentage. More {001} facet exposure could induce the generation of stronger IEF, which favored the photogenerated charge separation an...