An electroneutral Na-HCO(3)(-) cotransporter (NBC(N)1) was recently cloned, and Northern blot analyses indicated its expression in rat kidney. In this study, we determined the cellular and subcellular localization of NBC(N)1 in the rat kidney at the light and electron microscopic level. A peptide-derived antibody was raised against the COOH-terminal amino acids of NBC(N)1. The affinity-purified...

An NbC-Fe composite powder was synthesized from an Nb₂O₅/Fe/C mixture by electrochemical reduction and subsequent carbonization in molten CaCl₂-NaCl. The composite has a core-shell structure, in which NbC acts as the cores distributing in the Fe matrix. A strong bonding between NbC and Fe is benefit from the core-shell structure. The sintering and electrochemical reduction processes were invest...

Starting from a high-order local nonreflecting boundary condition (NBC) for single scattering [25], we derive a local NBC for time-dependent multiple scattering problems, which is completely local both in space and time. To do so, we first develop an exterior evaluation formula for a purely outgoing wave field, given its values and those of certain auxiliary functions needed for the local NBC a...

The CrS/NbC Co-based self-lubricating composite coatings were successfully fabricated on Cr12MoV steel surface by laser clad Stellite 6, WS₂, and NbC mixed powders. The phase composition, microstructure, and tribological properties of the coatings ware investigated by means of X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy dispersive spectrometer (EDS), as well as dry s...

The formation of carbon nanotube and superconductor composites makes it possible to produce new and/or improved functionalities that the individual material does not possess. Here we show that coating carbon nanotube forests with superconducting niobium carbide (NbC) does not destroy the microstructure of the nanotubes. NbC also shows much improved superconducting properties such as a higher ir...

Salivary glands secrete K(+) and HCO(-)(3) and reabsorb Na(+) and Cl(-), but the identity of transporters involved in HCO(-)(3) transport remains unclear. We investigated localization of Cl(-)/HCO(-)(3) exchanger isoform AE2 and of Na(+)-HCO(-)(3) cotransporter (NBC) in rat parotid gland (PAR) and submandibular gland (SMG) by immunoblot and immunocytochemical techniques. Immunoblotting of PAR a...

Electrogenic cotransport of Na+ and HCO-3 is a crucial element of HCO-3 reabsorption in the renal proximal tubule (PT). An electrogenic Na+-HCO-3 cotransporter (NBC) has recently been cloned from salamander and rat kidney. In the present study, we generated polyclonal antibodies (pAbs) to NBC and used them to characterize NBC on the protein level by immunochemical methods. We generated pAbs in ...

Ruiz, Ofelia S., R. Brooks Robey, Yi-Yong Qiu, Long Jiang Wang, Cheng Jin Li, Jianfei Ma, and Jose A. L. Arruda. Regulation of the renal Na-HCO3 cotransporter. XI. Signal transduction underlying CO2 stimulation. Am. J. Physiol. 277 (Renal Physiol. 46): F580–F586, 1999.—We have previously shown that CO2 stimulation of the renal Na-HCO3 cotransporter (NBC) activity is abrogated by general inhibit...

PURPOSE To measure tear film surface quality in healthy and dry eye subjects using three noninvasive techniques of tear film quality assessment and to establish the ability of these noninvasive techniques to predict dry eye. METHODS Thirty-four subjects participated in the study and were classified as dry eye or normal, based on standard clinical assessments. Three noninvasive techniques were...

Epitaxial NbC thin films were grown by a chemical solution technique, polymer assisted deposition. High quality epitaxial NbC film showed a transition temperature of 10 K and a hardness of 19.54 GPa.