Infrared spectroscopy has been widely applied for the characterisation of various substances. Due to its sensitivity to the chemical information and architecture of the molecule, infrared spectroscopy can play an important role in new applications such as in the life-science field and not only in the traditional fields of physics and chemistry. Spectroscopic techniques are simple, reproducible,...

High-definition Fourier Transform Infrared (FT-IR) spectroscopic imaging is an emerging approach to obtain detailed images that have associated biochemical information. FT-IR imaging of tissue is based on the principle that different regions of the mid-infrared are absorbed by different chemical bonds (e.g., C=O, C-H, N-H) within cells or tissue that can then be related to the presence and comp...

BACKGROUND Fourier transform infrared (FT-IR) spectroscopy is an effective tool for investigation of chemical changes at the molecular level. We previously demonstrated that FT-IR spectroscopy can reliably distinguish multiple types of carcinoma from healthy tissue. Because various stomach diseases are common, it is important to explore a noninvasive and rapid method to detect malignancy and ga...

FT-IR spectra of liver tissue isolated from mice, Mus musculus, have been recorded in the region of 4000 – 400 cm-1 for normal, mercury treated and recovery phase. In this study, the total protein content was found to be decreased in the liver tissues after treatment with median-lethal dose of mercuric chloride. The marked fall in the level of bio-chemical constituent in the tissue due to metal...

Fourier transform infrared (FT-IR) spectroscopy is a physico-chemical method based on measurement of vibration of a molecule excited by IR radiation at a specific wavelength range. FT-IR spectra of bacterial cells can be used to analyze their total composition, including proteins, fatty acids, carbohydrates, nucleic acids, and lipopolysacharides. FT-IR techniques coupled with different chemomet...

21 clinical isolates of Candida spp were examined by FT-IR in order to compare identification using FT-IR with biochemical identification (bioMerieux ® ). FT-IR revealed itself as a fast and effective method for identification of the strains tested as all were placed into the appropriate species, including one which was misidentified by the biochemical method.

BACKGROUND Fourier-transform infrared (FT-IR) spectrometry has been used to measure small molecules in plasma. We wished to extend this use to measurement of plasma proteins. METHODS We analyzed plasma proteins, glucose, lactate, and urea in 49 blood samples from 35 healthy subjects and 14 patients. For determining the concentration of each biomolecule, the method used the following steps: (a...

AIMS Fourier transform infrared (FT-IR) was used to analyse a selection of Acinetobacter isolates in order to determine if this approach could discriminate readily between the known genomic species of this genus and environmental isolates from activated sludge. METHODS AND RESULTS FT-IR spectroscopy is a rapid whole-organism fingerprinting method, typically taking only 10 s per sample, and ge...

The present study was designed to investigate the potential of Fourier transform infrared (FT-IR) spectroscopy to identify Trueperella (T.) pyogenes isolated from bovine clinical mastitis. FT-IR spectroscopy was applied to 57 isolates obtained from 55 cows in a period from 2009 to 2012. Prior to FT-IR spectroscopy these isolates were identified by phenotypic and genotypic properties, also inclu...

We have investigated the ordinate scale accuracy of ambient temperature transmittance measurements made with a Fourier transform infrared (FT-IR) spectrophotometer over the wavelength range of 2-10 mum. Two approaches are used: (1) measurements of Si wafers whose index of refraction are well known from 2 to 5 mum, in which case the FT-IR result is compared with calculated values; (2) comparison...