DTS-II is a highly diphtheria toxin (DT)-sensitive cell line previously isolated by transfection of wild-type DT-resistant mouse L-M(TK-) cells with the cDNA encoding a monkey Vero cell DT receptor. DTS-II cells are as toxin-sensitive as Vero cells, have approximately 3-fold more receptors than Vero cells, and have approximately 10-fold lower affinity for DT than Vero cells. We now cotransfecte...

Plaque formation in Vero cells has been induced with seven rabies serogroup viruses either cocultivated as infected BHK-21 or Aedes albopictus cells, or directly cultivated as infected mouse brain.

measles has been a major cause of illness and death in children and vaccination against the disease is part of the who global immunization program. a suitable vaccine should create maximum immune response against the pathogen and must be safe for the user. thus, after production, vaccines must be analyzed and controlled by the producer and confirm by relevant governmental organizations. the foo...

poliomyelitis, an acute viral infectious disease caused by poliovirus, still remains a public health problem in developing countries. despite the global effort to eradicate polio, continuing the polio immunization with a potent and safe vaccine is essential. for accurate vaccine evaluation, three types of cell lines including hela, hep2c and vero were evaluated and compared using two methods of...

This work investigated the replication kinetics of the four dengue virus serotypes (DEN-1 to DEN-4), including dengue virus type 4 (DEN-4) recovered from an infectious cDNA clone, in Vero cells and in MRC-5 cells grown on Cytodex 1 microcarriers. DEN-1 strain Hawaii, DEN-2 strain NGC, DEN-3 strain H-87, and DEN-4 strain H-241 , and DEN-4 strain 814669 derived from cloned DNA, were used to infec...

abstract: peganum harmala l. (zygophylaceae) has been used in a variety of practical applications in medical science. our objective in the current study was to determine the effects of the seed extract on herpes simplex virus type 1 (hsv-1) replication in vero cells. different concentrations (100, 500, 571, 667, 800 and 1000 µg/ml) of the extract were examined. experiments were carried out usin...

BACKGROUND Wild-type measles virus (MV) is isolated in B95a but not in Vero cells. Through an adaptation process of wild-type MV to Vero cells, several amino acid substitutions were reported. METHODS Six strains were adapted to Vero cells and membrane (M), fusion (F) and hemagglutinin (H) genes were sequenced. Cell fusion was assessed and recombinant MVs were constructed, having wild-type H o...

The role of antibiotics in the treatment of Shiga-like toxin (Stx)-producing E. coli infection is still controversial. This study investigated the effects of colistin on Vero cell cytotoxicity caused by the enterohemorrhagic EC O157:H7, and the effects of colistin on Stx and endotoxin release by EC O157:H7. Vero cells were incubated with supernatant collected from EC O157:H7 cultured for 18 h w...

Heat shock proteins (HSP) have been identified as an important factor of a very complex and highly conserved cellular defense mechanism to preserve cell survival under adverse environmental conditions. HSP 60 are immunodominant antigens of microbe such as Chlamydia trachomatis and have a potentiality to become a target antigen due to antigenic similarity between chlamydial and human HSP. This s...