BACKGROUND/AIMS A(3) adenosine receptor mediates apoptosis in a variety of cancer cells via diverse signaling pathways. The present study was conducted to assess A(3) adenosine receptor-mediated apoptosis in human bladder cancer cell lines and to understand the underlying mechanism. METHODS Human bladder cancer cell lines such as 253J, 5637, KK-47, TCCSUP, T24, and UMUC-3 cells were cultured....

Here we address the molecular mechanism of serum-independent survival and growth of human bladder carcinoma cell line 5637. Serum starvation promoted tyrosine phosphorylation of a 145-kDa protein and activation of the tyrosine kinase Src and the receptor for epidermal growth factor (EGFR) over a slow time course (>8 hours). The phosphorylated 145-kDa protein was identified as the beta-subunit o...

Abstract Objectives The aim of this study was to investigate the effect lncRNA-SNHG15 in bladder carcinoma using cell lines experiments and relationship between clinical characteristics expression analyzed. Methods Bladder cancer tissues near-cancer were collected. real-time PCR (RT-PCR) used detect lines. downregulated by interference (siRNA), as detected RT-PCR, that determine efficiency inte...

Objective: To investigate the inhibitory effect of metformin on bladder cancer cell and its mechanism. Methods: In this study, metformin with different concentrations (0, 2, 5, 10, 20 mM) was used for the intervention on bladder cancer cell lines 5637 and T24 in vitro. Its influence on the growth and proliferation of cells was detected by MTT assay; the impact on cell colony formation was quant...

Resveratrol, an edible polyphenolic phytoalexin present in grapes and red wine, has been reported to inhibit proliferation and induce apoptosis of bladder cancer cells. In the present study, the molecular mechanisms of resveratrol on human bladder cancer cell apoptosis was examined. The effect of resveratrol on the viability and apoptosis of T24 and 5637 cells was measured using an MTT assay an...

BACKGROUND Xeroderma pigmentosum group F (XPF) is an important participant in the nucleotide excision repair process. This study aimed to investigate the expression of DNA repair gene xeroderma pigmentosum group F (XPF) in bladder cancer and its clinical significance. MATERIAL/METHODS Total RNA and protein were extracted from 45 untreated bladder cancer tissues and 21 hydroxycamptothecin (HCP...

BACKGROUND The aim of this study was to identify key genes linked to the molecular action of gefitinib, a promising anticancer agent on human bladder cancer cell lines. MATERIALS AND METHODS cDNA microarrays were used to profile feature genes in 5637 and T24 cells before and after treatment with gefitinib. PCR-based direct sequencing and Western blot analysis were performed to examine the mut...