Methods: The studied population included 59 MDRAB strains previously genotyped by PFGE and multilocus sequence typing. The search for the ADC (blaADC), CHO (blaOXA-51-like, blaOXA-23-like, blaOXA-40-like and blaOXA-58-like) and MBL (blaIMP, blaVIM, blaSIM-1, blaGIM-1, blaSPM-1 and blaNDM-1) genes, and for the ISs (ISAba1, ISAba2, ISAba3, ISAba4 and IS18) was done by PCR assays. The phenotypic p...

A microdilution test measuring imipenem MICs in the presence or absence of a mixture of EDTA plus 1,10-phenanthroline was developed and tested on 190 Pseudomonas aeruginosa isolates, including 18 VIM- and 4 IMP-type metallo-beta-lactamase (MBL) producers. The chelator mixture reduced by fourfold or more the imipenem MICs for MBL producers, while a lower effect or no effect was usually observed ...

background and objectives : carbapenem resistant pseudomonas aeruginosa is a serious cause of nosocomial infections.the main purpose of the study is to determine the prevalence rate of imipenem resistant pseudomonas aeruginosa carrying metallo- beta- lactamase (mbl) genes. material and methods : 236 pseudomonas aeruginosa isolates were collected from teaching hospitals of ahvaz university of me...

OBJECTIVES To evaluate four phenotypic tests for the detection of metallo-beta-lactamase (MBL) production in Pseudomonas aeruginosa in a low MBL prevalence setting. METHODS Sixty clinical isolates of P. aeruginosa resistant to imipenem and/or meropenem and seven MBL-positive control strains were examined by: (i) MBL Etest; (ii) combined imipenem discs supplemented with EDTA (IPM-EDTA); (iii) ...

Prompt detection of metallo-beta-lactamase (MBL) producing isolates is necessary to prevent their dissemination. Frequency of MBLs producing strains among multidrug resistant (MDR) Acinetobacter species and Pseudomonas aeruginosa was evaluated in critical care patients using imipenem-EDTA disk method. One hundred MDR Acinetobacter spp. and 42 Pseudomonas aeruginosa were checked for MBL producti...

Background and Objectives New Delhi metallo-ß-lactamase (NDM) is a newly emerging metallo-ß-lactamases, which can destroy all β-lactams including carbapenems. Therefore, this study aimed at evaluating New Delhi metallo-ß-lactamase-1-production in clinical isolates of Klebsiella pneumoniae in Kashan, Iran. Materials and Methods In a cross-sectional study, 181 K. pneumoniae isolates were collec...

Pseudomonas aeruginosa, an important opportunistic pathogen causing a wide range of acute and chronic infections.The present study was undertaken to detect the Metallo β lactamase in isolated of Pseudomonas aeruginosa which was isolated from wound infection and evaluated their susceptibility patterns.The presence of the MBL enzyme was detected by phenotypic confirmatiory tests are IMIPENEM-EDTA...

The current phenotypic methods for detecting carbapenemase-producing Enterobacteriaceae (CPE) allow differentiation between class A and B carbapenemases, but they cannot confirm in a single test class D OXA-48 carbapenemase producers. In this study, we evaluated a new phenotypic test, the OXA-48 disk test, which is based on an imipenem disk and two blank disks adjacent to the imipenem disk, loa...

Nonfermenting gram negative bacilli (NFGNB) cause serious infections in immuncompromised and hospitalized patients. They cause fatal lower respiratory tract infections in patients admitted to ICU’s. The appearance of metallo ß-lactamases (MBL) genes and their spread among bacterial pathogens is a matter of concern. The present study was undertaken to know the prevalence of MBL producing NFGNB.N...