SARS-CoV-2 was the cause of global COVID-19 pandemic in 2019. The cycle threshold (CT) value real-time RT-PCR technique is gold standard infections diagnosis, risk assessment, discharged criteria, and patient isolation for SARS-CoV-2. Heat inactivation a simple, economical solution safety testing change CT values after heat has not been thoroughly evaluated. This study evaluated effect on realt...

Virus dịch tả lợn Châu Phi (ASFV) và virus cổ điển (CSFV) đã gây thiệt hại kinh tế rất lớn cho người chăn nuôi lợn. Có nhiều phương pháp xét nghiệm để phát hiện hai này như: PCR, Realtime phân lập virus, ELISA. Bên cạnh đó Multiplex Real-time RT-PCR (mReal-time RT-PCR) đồng thời ASFV CSFV cũng được đánh giá ở một số nước trên thế giới nhưng chưa nghiên cứu tại Việt Nam. Trong chúng tôi mReal-ti...

INTRODUCTION The Abbott RealTime is a novel real-time PCR assay designed for concurrent individual genotyping of HPV16 and HPV18 and pooled detection of 12 HPV genotypes: HPV31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68 in cervical swab specimens. In this study, the performance of RealTime for detecting HPV in formalin-fixed, paraffin-embedded tissue specimens of head and neck cancers was ...

which excluded false-positive results because of amplicon contamination. The second case was identifi ed among the 86 sheep investigated. Placenta and fetal lung and liver exhibited necrotizing placentitis and vasculitis (Figure, panel A), interstitial pneumonia (Figure, panel B), and mixed cellular periportal hepatitis. Fetal liver was negative by parachlamydial 16S rRNA real-time PCR and immu...

Presence of Mycobacterium avium subsp. paratuberculosis (MAP) in beef has been reported as a public health concern because asymptomatically infected cattle may contain MAP in tissues that are used for human consumption. Associations between MAP carcasses contamination and animal characteristics such as age, breed, production type, and carcass classification were assessed. Cheek muscles from 501...

The paper describes a developed comprehensive system for molecular-genetic identification of Yersinia pestis strains according to their appurtenance certain subspecies, biovars, phylo-geographic populations, using realtime PCR (RT-PCR), allele-specific RT-PCR, and multiplex with hybridization fluorescent registration results on solid substrate. Application this makes it possible establish the Y...

The Abbott RealTime HCV Genotype II kit is designed for use in the transcription and amplification of the 5’UTR or NS5B regions of the hepatitis C virus (HCV) genome. RealTime-PCR amplification is conducted in three separate reaction mixtures with different fluorophores where genotypespecific probes are used to detect HCV genotypes 1, 1a, 1b, 2, 3, 4, 5 and 6 such that: • Reaction A detects all...