results the optimal conditions were considered to perform elisa. comparison between results obtained from optimized elisa kit and commercial elisa kit showed a good agreement. background haemophilus influenzae type b (hib) infection has high morbidity and mortality rate, especially in children under 5 years of age. enzyme-linked immunosorbent assay (elisa) technique is the most used method to d...

background: retroviruses of human t-lymphotropic viruses (htlv-1 and htlv-2) have been demonstrated to be endemic in the north-eastern region of iran. this study aims to determine htlv-1 and htlv-2 prevalence among healthy individuals in neyshabur city during 2010-2014. methods: a total of 8054 blood samples were collected from healthy participants in neyshabur, north-eastern iran. the blood sa...

results 34 (34%) of the renal transplant recipients and 26 (26%) of the control group were positive for anti-toxoplasma igg. in addition 18 (18%) of the renal transplant recipients and 4 (4%) of the control group were positive for anti-toxoplasma igm. for both values of the antibodies, differences were statistically significant (p < 0.025 and p < 0.001 respectively). the pcr results indicated t...

conclusions the present study supported the contamination with t. gondii as a risk factor for schizophrenia just in women. results the analyses confirmed the significant differences between healthy women and ones with schizophrenia (p = 0.001) as well as between males and females with schizophrenia (p = 0.009) in igg positivity. materials and methods t. gondii igg antibodies of 100 patients wit...

background there are different types of aflatoxins, including aflatoxin m1, m2, g1, g2, b1 and b2 produced in the food chain, especially when the food is infected with fungi and can directly be consumed by humans. considering that our country is located in the subtropical geographic region where temperature and humidity ecological factors are among determining factors in contamination with afm1...

background: diarrhea, caused by enteroaggregative escherichia coli (eaec), is an important cause of illness and death. numerous virulent factors have been described in eaec. however, their prevalence was highly variable among eaecs of distinct geographic locations. studies have shown that dispersin (antiaggregation protein, aap) is one of the important and abundant virulent factors in eaec. in ...

introduction: periodontitis is a multi-factorial inflammatory disease of periodontium. a precise mechanism in which smoking affects periodontium is not accurately understood. this study was planned to survey salivary osteocalcin (oc) level among smokers with chronic periodontitis and its comparison to non-smoker patients which may help achieve a better understanding of mechanisms being involved...

An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assa...

there are many studies related to the production of a elisa kit for diagnosing virus infections. however, production of most kits depends on purification of whole virus particles, which involves the use of costly equipment and reagents. the purpose of this study was to check out if the anti-cp42 antibodies could be used as a diagnostic assay for detection of grapevine fanleaf virus (gflv). in t...

diagnosis of toxoplasma gondii (t.gondii) infection is of great medical importance especially for pregnant women and immunosuppressed patients. numerous studies have shown that the recombinant production of several ttoxoplasma antigens, including dense granule antigens (gras) has a great potential as diagnostic reagents. previous studies reported expression of amino terminal gra8 protein in fus...