conclusions the study indicated that the rate of p. jirovecii infection is similar to those reported from asian, indian and african countries. the similarity of the genetic pattern between iranian and indian isolates is probably due to their close geographical proximity. patients and methods we used two nested pcr and pcr-rflp assays to amplify mt lsu rrna and dhps genes in 126 samples obtained...

cryptosporidiosis is a gastroenteric disease caused by the protozoan parasite cryptosporidium parvum. water–borne transmission of this organism has become more prevalent in recent years. current method for detection of c. parvum oocysts in water is immunofluoresence assay (ifa). the method is time consuming, laborious and particularly not–specific. it cannot determine the infectivity of detecte...

objective: hepatitis c virus is the major cause of viral hepatitis and its diagnosis in suspected specimens is of great importance. the risk of transfusion- transmitted virus infection is primarily the result of failure in serological screening tests to detect recently infected donors in the pre-seroconversion window period of infection. therefore, sensitive and accurate diagnosis of hcv prior ...

Check out our Selection Guide! PCR RNA DNA LAMP KitsOne Tube For Superior Reproducibility. From fish tissues or fluids using a nested PCR primer set. The use of trade, firm, or corporation names in this protocol is for the information and.jor consideration in designing a nested PCR protocol compatible with quantitation is to assure that the maximum concentration of PCR products produced by the....

a nested polymerase chain reaction (pcr) assay with two pairs of primers was applied for the detection of m. tuberculosis complex dna in specimens of 51 patients. of these, 31 clinically diagnosed patients (group i) recruited from july 1996 to june 1997 were given antituherculous chemotherapy from one to six months with standard daily regimen (isaniazid, 5 mg/kg; rifampin, 10 mg/kg; pyrazinamit...