Background and Objective: Recent studies have shown that anticholinergic alkaloids compounds have strong analgesic effects. Due to presence of anticholinergic alkaloids in Hyoscyamus niger and its mentioned effects in Iranian traditional medicine, its analgesic effects were assessed. At first, acute and chronic pain thresholds in male NMRI rats with formalin test was evaluated, then oral and in...

Various plant organ segments including segments from roots (root tip, and root collar), stems, leaves, and also seeds of several Hyoscyamus species (i.e. H. pusillus, H. arachnoideus, annual and biennial H. niger from two distinct geographical regions) were cultured on MS media with various growth regulator combinations, to assess the ability of flower production. Statistical assessments showed...

In the biosynthesis of tropane alkaloids, two tropinone reductases (TRs) catalyze reduction of tropinone to different stereoisomers, tropine and pseudotropine. Two TRs from Hyoscyamus niger have 64% of identical amino acids and hence a common evolutionary origin. In this study, genomic clones of TRs were isolated from H. niger. Their sequence comparison showed that although they have the same e...

Analysis of genes preferentially expressed in hairy roots caused by infection with Agrobacterium rhizogenes has provided insights into the regulation of lateral root formation. A hairy root preferential cDNA, HR7, has been cloned from hairy roots of Hyoscyamus niger. HR7 encodes a novel protein partially homologous to a metallocarboxypeptidase inhibitor and is expressed exclusively in the primo...

background: since alkaloids are nitrogenous compounds, the availability of nitrogen (n) is expected to play an important role in the biosynthesis and accumulation of alkaloids in plants. objective: this study intended to investigate the nitrogen (n) fertilization and water deficit stress (wds) effects on seed tropane alkaloids elicitation including hyoscyamine (hyo) and scopolamine (sco), and a...