Download pose: Phosphorylated histone H2AX (γH2AX) serves as a biomarker for formation of DNA doublebreak repair complexes. A quantitative pharmacodynamic immunofluorescence assay for γH2AX eveloped, validated, and tested in human tumor xenograft models with the use of clinically relevant ures. erimental Design: The γH2AX immunofluorescence assay uses a novel data quantitation and imocessing al...

BACKGROUND γH2AX is a protein biomarker for double-stranded DNA breakage; its expression was studied in cervical squamous intraepithelial lesions and carcinomas. METHODS Immunostaining for phospho-γH2AX was performed in sections from histologically confirmed cervical SIL and carcinomas, as well as from normal cervices used as controls. In total, 275 cases were included in the study: 112 low g...

The phosphorylation of H2Ax on its S139 site, γH2Ax, is important during DNA double-strand repair and is considered necessary for assembly of repair complexes, but its functional role after other kinds of DNA damage is less clear. We have measured the survival of isogenic mouse cell lines with the H2Ax gene knocked out, and replaced with wild-type or mutant (S139A) H2Ax genes, exposed to a rang...

DNA damage response (DDR) molecules are protective against genotoxic stresses. DDR molecules are also involved in the survival of cancer cells in patients undergoing anti-cancer therapies. Therefore, DDR molecules are potential markers of cancer progression in addition to being potential therapeutic targets. In this study, we evaluated the immunohistochemical expression of PARP1, γH2AX, BRCA1, ...

Downloa pose: Phosphorylated histone H2AX (γH2AX) serves as a biomarker for formation of DNA doublebreak repair complexes. A quantitative pharmacodynamic immunofluorescence assay for γH2AX eveloped, validated, and tested in human tumor xenograft models with the use of clinically relevant ures. erimental Design: The γH2AX immunofluorescence assay uses a novel data quantitation and imocessing alg...

Phosphorylation of the histone H2AX (γH2AX form) is an early response to DNA damage and a marker of aging and disease in several cells and tissues outside the nervous system. Little is known about in vivo phosphorylation of H2AX in neurons, although it was suggested that γH2AX is an early marker of neuronal endangerment thus opening the possibility to target it as a neuroprotective strategy. Af...

cells but can be deadly. A single unrepaired DSB will kill a yeast cell deficient in recombination [1]. Unrepaired DSBs lead to chromosome damage and are associated with aging and cancer. Until a few years ago, methods used for their detection relied exclusively on physical techniques such as pulsed field gel electrophoresis that measure changes in the size of DNA molecules. Unfortunately, thes...

BACKGROUND Poly(ADP-ribose) polymerase 1 (PARP1), γH2AX, BRCA1, and BRCA2 are conventional molecular indicators of DNA damage in cells and are often overexpressed in various cancers. In this study, we aimed, using immunohistochemical detection, whether the co-expression of PARP1, γH2AX, BRCA1, and BRCA2 in breast carcinoma (BCA) tissue can provide more reliable prediction of survival of BCA pat...

γH2AX has emerged in the last 20 years as a central player in the DDR (DNA damage response), with specificity for DSBs (double-strand breaks). Upon the generation of DSBs, γ-phosphorylation extends along megabase-long domains in chromatin, both sides of the damage. The significance of this mechanism is of great importance; it depicts a biological amplification mechanism where one DSB induces th...