Botrytis aclada and allii, associated with onion gray-mold neck rot isolated in Hokkaido, were tested for sensitivity to benzimidazole. Of the B. strains, 59% highly resistant remaining 41% sensitive; all strains of allii sensitive. Resistant widespread Hokkaido. We analyzed sequences β-tubulin gene detected replacement glutamic acid (GAG) by lysine (AAG) at codon 198. This is first report benz...

This work aimed to identify the species distribution of common clinical and environmental isolates of black Aspergilli based on simple restriction fragment length polymorphism (RFLP) analysis of the β-tubulin gene. A total of 149 clinical and environmental strains of black Aspergilli were collected and subjected to preliminary morphological examination. Total genomic DNAs were extracted, and PC...

Malignant melanoma might develop from melanocytic nevi in which the growth-arrested state has been broken. We analyzed the gene expression of young and senescent human melanocytes in culture and compared the gene expression data with a dataset from nevi and melanomas. A concordant altered gene expression was identified in 84 genes when comparing the growth-arrested samples with proliferating sa...

during spring and summer of 2010 and 2012, several surveys were conducted in different vineyards in kohgiluyeh & boyer-ahmad (southwestern iran) and kerman (southeastern iran) provinces, with the aim of isolating and identifying fungi associated with grapevine decline diseases. the samples collected comprised diseased grapevines showing yellowing, defoliation, reduced growth, wilting, intervein...

b ackground: due to the lack of a suitable and economic test for the analysis of the polymorphism at codon 167, we developed a new pcr-rflp technique, based on a modified forward primer (ut- hc167 mf-primer), to identify simultaneously the snps at codons 167 and 200 of isotype 1 β-tubu- lin gene of haemonchus contortus . m ethods: there already are several safe and easy methods for identificati...

β-tubulins are structural components of microtubules and the targets of benzimidazole fungicides used to control many diseases of agricultural importance. Intron polymorphisms in the intron-rich genes of these proteins have been used in phylogeographic investigations of phytopathogenic fungi. In this work, we sequenced 2764 nucleotides of the β-tubulin gene (Pp tubB) in samples of Phakopsora pa...

In this study, a total of 300 isolates of Penicillium and related teleomorphic genera were collected from soils of 17 locations in Korea from April to May, 2004. Ninety four isolates were identified as the species of Penicillium subgenus Furcatum based on cultural and morphological characteristics and β-tubulin gene sequences. Among the species, Korean isolates of P. brasilianum Bat. and P. dal...

In the present study, a phylogenetic analysis was undertaken based on the internal transcribed spacer (ITS) rDNA and partial β-tubulin gene sequence of the Ganoderma species. The size of the ITS rDNA regions from different Ganoderma species varied from 625 to 673 bp, and those of the partial β-tubulin gene sequence were 419 bp. Based on the results, a phylogenetic tree was prepared which reveal...

The structure of the unique bacterial tubulin BtubA/B from Prosthecobacter is very similar to eukaryotic αβ-tubulin but, strikingly, BtubA/B fold without eukaryotic chaperones. Our sequence comparisons indicate that BtubA and BtubB do not really correspond to either α- or β-tubulin but have mosaic sequences with intertwining features from both. Their nucleotide-binding loops are more conserved,...