Apple (Malus × domestica) accumulates bioactive ursane-, oleanane-, and lupane-type triterpenes in its fruit cuticle, but their biosynthetic pathway is still poorly understood. We used a homology-based approach to identify and functionally characterize two new oxidosqualene cyclases (MdOSC4 and MdOSC5) and one cytochrome P450 (CYP716A175). The gene expression patterns of these enzymes and of pr...

Protium is the main genus of the Burseraceae family and one of the most common genera in South America, with an important species called "breu." Gum and oil-resins of this species are used as tonic and stimulant and for the treatment of ulcers and inflammation. The present study aims to isolate and investigate the anti-inflammatory activity of triterpene compounds isolated from oil-resin of Pro...

The essential oil of Salvia aegyptiaca was analysed by GC/ MS technique. Eighteen compounds were identified. It was found that the essential oil comprises 40% terpenoidal constituents, opposite to 32% fat derivatives. The major terpenoidal component was 1(10)aristolene (19.3%). From the non-volatile matter, β-amyrin, lupeol, β-sitosterol, stigmasterol, β-sitosterol-β-D-glucopyranoside and stigm...

Centella asiatica is a well-known medicinal plant, produces large amount of triterpenoid saponins, collectively known as centelloids, with a wide-spectrum of pharmacological applications. Various strategies have been developed for the production of plant secondary metabolites in cell and tissue cultures; one of these is modular metabolic engineering, in which one of the competitive metabolic pa...

Platostoma rotundifolium (Briq.) A. J. Paton aerial parts are widely used in Burundi traditional medicine to treat infectious diseases. In order to investigate their probable antibacterial activities, crude extracts from P. rotundifolium were assessed for their bactericidal and anti-virulence properties against an opportunistic bacterial model, Pseudomonas aeruginosa PAO1. Whereas none of the t...

BACKGROUND Metabolic engineering is an attractive approach in order to improve the microbial production of drugs. Triterpenes is a chemically diverse class of compounds and many among them are of interest from a human health perspective. A systematic experimental or computational survey of all feasible gene modifications to determine the genotype yielding the optimal triterpene production pheno...

Cirsium arvense (L.) Scop., commonly known as creeping thistle, is a weed of Asteraceae. This study was undertaken to explore various phytoconstituents present in flower this weed. To achieve goal, the dried flowers were soaked methanol for one week and filtered. methanolic extract subjected GC-MS analysis 7 compounds identified. These included olean-12-en-3-ol, acetate, (3β)- (63.87%), lanosta...

Abstract Antifungal activity of Monotheca buxifolia methanolic extract and its various fractions were assessed against Macrophomina phaseolina, a soil-borne fungal pathogen more than 500 vegetal species as well rare emerging opportunistic human pathogen. Different concentrations (3.125 to 200 mg mL −1 ) inhibited biomass by 39–45%. Isolated n- hexane, chloroform ethyl acetate suppressed 32–52%,...

This work describes the phytochemical study of the extracts from aerial parts of Tibouchina candolleana as well as the evaluation of the antimicrobial activity of extracts, isolated compounds, and semi-synthetic derivatives of ursolic acid against endodontic bacteria. HRGC analysis of the n-hexane extract of T. candolleana allowed identification of β-amyrin, α-amyrin, and β-sitosterol as major ...