An HPLC method for determination of αand γ-tocopherol in rat blood has been optimized and validated. The optimum means of separation and analysis of tocopherol homologs were selected on the basis of procedures found in the literature; documentary evidence is presented which shows precision and accuracy were satisfactory. Spectrofluorimetric detection was performed after separation on a Separon ...

BACKGROUND Vitamin E compounds exhibit prostate cancer preventive properties experimentally, but serologic investigations of tocopherols, and randomized controlled trials of supplementation in particular, have been inconsistent. Many studies suggest protective effects among smokers and for aggressive prostate cancer, however. METHODS We conducted a nested case-control study of serum α-tocophe...

The aim of this work was to compare protective and anti-apoptotic effects of α-tocopherol at nanomolar and micromolar concentrations against 0.2 mM H(2)O(2)-induced toxicity in the PC12 neuronal cell line and to reveal protein kinases that contribute to α-tocopherol protective action. The protection by 100 nM α-tocopherol against H(2)O(2)-induced PC12 cell death was pronounced if the time of pr...

α-Tocopherol (vitamin E) is an essential dietary antioxidant with important neuroprotective functions. α-Tocopherol deficiency manifests primarily in neurological pathologies, notably cerebellar dysfunctions such as spinocerebellar ataxia. To study the roles of α-tocopherol in the cerebellum, we used the α-tocopherol transfer protein for the murine version (Ttpa(-/)(-)) mice which lack the α-to...

OBJECTIVE To evaluate and compare the levels of α-tocopherol in colostrum and in the serum of healthy and diabetic mothers. METHODS This cross-sectional study enrolled 51 volunteer mothers, 20 with the diagnosis of gestational diabetes mellitus and 31 without associated diseases. Serum and colostrum samples were collected in fasting in the immediate postpartum period and α-tocopherol was anal...

Modern hip and knee endoprosthesis are produced in titanium and to reduce the friction at the contact area polymer parts, mainly ultra-high molecular weight polyethylene (UHMW-PE), are installed. The polyethylene is impregnated with αtocopherol (vitamin E) before processing for remarkable decrease of oxidative degradation. Cross linked UHMW-PE offers much higher stability, but α-tocopherol cann...

The study was to investigate the supplemental effect of α-tocopherol, β-carotene or combination of both on antioxidant capacities in plasma, liver and longissimus dorsi muscle (LM), and meat quality of pigs fed a high-linseed oil diet during the final finishing phase. Forty Duroc×Yorkshire×Landrace growing pigs were allocated to four high-linseed oil (3%) experimental diets formulated as basal ...

A new colorimetric method is proposed to determine α-tocopherol and its anti-oxidant activity by monitoring the ability of α-tocopherol to cause quantitative reduction of ferric ions. The method, based on Trinder reaction, includes incubating 1 μmol of ferric chloride in presence of 0 and 15 through 120 μmoles of α-tocopherol followed by detection of the iron (III) with standard mass of salicyl...

Vitamin E (α-tocopherol) is a natural antioxidant very useful for preventing the harmful effects of UV sun rays as skin aging and cancers. In this study, different MLV formulations were made using egg lecithin and varying molar ratios of α-tocopherol and/or cholesterol, and their encapsulation efficiencies were determined. The best liposomal product was incorporated into a carbomer 980 gel. The...